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Unformatted text preview: 1 Experiment 6-1: Standard Plate Count (Viable Count)(pgs 217-221) Purpose : To learn the methods for quantifying the amount of bacteria in a culture. Sometimes it is necessary to know the number of organisms in a culture for an experiment. There are several methods for accomplishing this: 1. Dilute the organisms and count them on microscopic Felds on a slide. 2. Quantitative Plating (Standard Plate Count) ones we 3. Turbidity measurement use Quantitative Plating (Serial Dilutions)- info on the viable organisms is obtained- easy to perform but uses lots of glassware and media and takes time to get results- can calculate the number of organisms in a bacterial culture- must use aseptic technique when handling the pipettes Volume added to the dilution blank Dilution actor(D)= Total volume in the tube (volume that was in the tube + volume added to the tube) inal dilution = product of individual dilutions number of colonies Original Cell Conc. = (volume plated) (dilution factor) CU = Colony orming Units (or the number of viable cells that can make a colony)...
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