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3-10 Hanging Drop & Motility

3-10 Hanging Drop & Motility - Experiment 3-10 Wet...

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1 Experiment 3-10: Wet Mount and Hanging Drop Preparations (pgs.100-101) Experiment 5-28: Motility Test (pgs. 198-199) Purpose : To learn how to determine if a bacterium is motile Movement: - Brownian motion : organism vibrates due to molecules hitting the cell, i.e. H 2 O, etc.; organism will remain in one place - true motility : directional; independent movement over greater distances -The direction of flagellar motion determines the nature of bacterial movement -ex. monotrichous polar flagella - flagella rotate counterclockwise - cell rotates clockwise - upon change of direction, the cell will tumble - chemotaxis - movement towards chemical attractants (ex. food) and away from repellants (ex. chemicals) When identifying bacteria, it is important to know if a bacteria is motile or not. Methods: Three Methods of Motility Determination : Wet Mount Hanging Drop Method Motility Test Media (semisolid media) Wet Mount : - Place two loopfuls of organism on a slide and cover with coverslip - Advantages of the Wet Mount :
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