1Experiment 3-11:Flagellar Staining(pgs 102-103)Purpose: To learn about flagella and how to stain themFlagellar Staining is typically performed using Leifson’sMethod:Procedure:1. Clean a slide and heat it in the blue portion of the flame.2. While the slide is still hot, mark with a china marking pencilas shown.3. Place a loopful of organism on the right side of the slide.4. Tilt the slide so that the organism flows down over thesurface of the slide5. Completely air-dry the slide. (DO NOT APPLY HEAT!!)6. Add Leifson’s stain to the slide and let the alcohol in thestain evaporate.7. Rinse gently with water to remove stain.8. Air dry and examine under oil immersion.Leifson’s method is difficult to do, so it is not used as much anymore. In its place, flagella aregenerally visualized with electron microscopy.Flagella are hard to see so it is necessary to increase the diameter in order to visualize themunder the microscope.
This is the end of the preview.
access the rest of the document.