3-11 Flagellar Stain

3-11 Flagellar Stain - 1 Experiment 3-11:Flagellar...

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Unformatted text preview: 1 Experiment 3-11:Flagellar Staining(pgs 102-103) Purpose : To learn about fagella and how to stain them Flagellar Staining is typically perormed using Leisons Method: Procedure: 1. Clean a slide and heat it in the blue portion o the fame. 2. While the slide is still hot, mark with a china marking pencil as shown. 3. Place a loopul o organism on the right side o the slide. 4. Tilt the slide so that the organism fows down over the surace o the slide 5. Completely air-dry the slide. (DO NOT APPLY HEAT!!) 6. Add Leisons stain to the slide and let the alcohol in the stain evaporate. 7. Rinse gently with water to remove stain. 8. Air dry and examine under oil immersion. Leisons method is dicult to do, so it is not used as much anymore. In its place, fagella are generally visualized with electron microscopy. Flagella are hard to see so it is necessary to increase the diameter in order to visualize them under the microscope....
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