Enzymes bio lab - Justin Jacques 12/6/07 TA: Austin...

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Justin Jacques 12/6/07 TA: Austin Stonebraker Biology 411 Lab Enzymes Introduction: In this lab, we will be focusing on the chemical structure of enzymes. Enzymes are proteins in the cell that control and manipulate cellular reactions. These proteins consist of a series of long chains of amino acids. When there is a structural change in enzymes, it destroys its ability to function. This process is known as denaturing. When enzymes lower the activation energy, it causes the metabolic reactions to accelerate. In this process, substrates bind to reacting molecules and form the enzyme-substrate complex. When the desired product is formed, the metabolic reaction is complete. There are a few things that can affect enzyme activity. An example includes temperature, which can dramatically affect metabolic rates. The reaction rate in enzyme activity can double with every 10C increase. PH can also affect enzyme activity. PH, which ranges from 1 – 14, is a measure of concentration between H+ and OH-. The PH measures between 1-6 are acidic, pH 7 is neutral, and pH 8-14 consists of a high concentration of OH- and is called Alkaline. Contributing ions known as buffers stabilizes PH. There are two types of enzyme that prevent the action of enzyme activity from completing. These two inhibitors are known as competitive inhibition and non- competitive inhibition. Competitive inhibition is when the peroxidase breaks down peroxide. Hydroxylamine, a molecule similar to peroxide, binds to peroxidase’s active, thus inhibiting peroxide binding. Non-competitive inhibition uses an allostreric inhibitor to bind to the enzyme’s active site and changes the shape of another active site.
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Justin Jacques 12/6/07 TA: Austin Stonebraker Biology 411 Lab In this lab, we will be working with a chemical known as catechol oxidase. Catechol oxidase is an enzyme that oxidizes catechol to benzoquinone. The purpose of this lab is to describe the structure and function of enzymes. We will also
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Enzymes bio lab - Justin Jacques 12/6/07 TA: Austin...

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