Enzymes bio lab - Justin Jacques TA Austin Stonebraker...

Info icon This preview shows pages 1–3. Sign up to view the full content.

View Full Document Right Arrow Icon
Justin Jacques 12/6/07 TA: Austin Stonebraker Biology 411 Lab Enzymes Introduction: In this lab, we will be focusing on the chemical structure of enzymes. Enzymes are proteins in the cell that control and manipulate cellular reactions. These proteins consist of a series of long chains of amino acids. When there is a structural change in enzymes, it destroys its ability to function. This process is known as denaturing. When enzymes lower the activation energy, it causes the metabolic reactions to accelerate. In this process, substrates bind to reacting molecules and form the enzyme-substrate complex. When the desired product is formed, the metabolic reaction is complete. There are a few things that can affect enzyme activity. An example includes temperature, which can dramatically affect metabolic rates. The reaction rate in enzyme activity can double with every 10C increase. PH can also affect enzyme activity. PH, which ranges from 1 – 14, is a measure of concentration between H+ and OH-. The PH measures between 1-6 are acidic, pH 7 is neutral, and pH 8-14 consists of a high concentration of OH- and is called Alkaline. Contributing ions known as buffers stabilizes PH. There are two types of enzyme that prevent the action of enzyme activity from completing. These two inhibitors are known as competitive inhibition and non- competitive inhibition. Competitive inhibition is when the peroxidase breaks down peroxide. Hydroxylamine, a molecule similar to peroxide, binds to peroxidase’s active, thus inhibiting peroxide binding. Non-competitive inhibition uses an allostreric inhibitor to bind to the enzyme’s active site and changes the shape of another active site.
Image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Justin Jacques 12/6/07 TA: Austin Stonebraker Biology 411 Lab In this lab, we will be working with a chemical known as catechol oxidase. Catechol oxidase is an enzyme that oxidizes catechol to benzoquinone.
Image of page 2
Image of page 3
This is the end of the preview. Sign up to access the rest of the document.

{[ snackBarMessage ]}

What students are saying

  • Left Quote Icon

    As a current student on this bumpy collegiate pathway, I stumbled upon Course Hero, where I can find study resources for nearly all my courses, get online help from tutors 24/7, and even share my old projects, papers, and lecture notes with other students.

    Student Picture

    Kiran Temple University Fox School of Business ‘17, Course Hero Intern

  • Left Quote Icon

    I cannot even describe how much Course Hero helped me this summer. It’s truly become something I can always rely on and help me. In the end, I was not only able to survive summer classes, but I was able to thrive thanks to Course Hero.

    Student Picture

    Dana University of Pennsylvania ‘17, Course Hero Intern

  • Left Quote Icon

    The ability to access any university’s resources through Course Hero proved invaluable in my case. I was behind on Tulane coursework and actually used UCLA’s materials to help me move forward and get everything together on time.

    Student Picture

    Jill Tulane University ‘16, Course Hero Intern