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Unformatted text preview: Zach Lashaway Monday, #81 9-22-07 Separation of Amino Acids by Chromatography Introduction: An effective way to separate amino acids from a mixture is by chromatography. Chromatography is a method by which a solvent (mobile phase) influences the separation of a mixture through migration of a stationary phase. The mobile phase (a solvent) migrates along the stationary phase allowing the mixture to be separated by way of differential migration. The stationary phase is stable and allows the solution to travel through it, thus showing the separation of substances. By analyzing the separation of the solution in the stationary phase, conclusion can be drawn about what exactly is in the solution. A common type of chromatography is paper chromatography. Paper chromatography is used to demonstrate the separation of a mixture (in this case amino acids) by way of filter paper and solution. In paper chromatography filter paper acts as a stationary phase while isopropyl alcohol in ammonia acts as a mobile phase. Once the solvent migrates through the paper while isopropyl alcohol in ammonia acts as a mobile phase....
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