Chem3AL-Lab5 - ThinLayerChromatography(TLC)

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Thin Layer Chromatography (TLC) First, our group spotted four TLC plates with aspirin, acetaminophen, caffeine, and ibuprofen. Then we each used one of the solvents, 100% ethyl acetate, 92:8 ethyl acetate:acetic acid, 60:40 hexane:ethyl acetate, or 10:10:1 toluene:ethyl acetate:acetic acid, to separate the compounds. To determine which two solvents to use in Part II, we examined the retention factor (Rf) values of the compounds on each plate. The greater the disparity between Rf values, the better the solvent for Part II. We came to the conclusion of using 92:8 ethyl acetate:acetic acid and 10:10:1 toluene:ethyl acetate:acetic acid after finding that these two solvents caused the greatest amount of separation between compounds. After we chose these two solvents for Part II, we divided the tasks according to Table 1 (back page) and ran the separation. Our unknown was identified relatively easily because the compound separated into three components on the plate and only one possible unknown was composed of three components, Excedrin. The spots of the
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Chem3AL-Lab5 - ThinLayerChromatography(TLC)

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