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Paper Chromatography:SEPATATION OF A MIXTURE USING PHYSICAL EQUILIBRIAREBECCA FORLOINELAB PARTNER: TERRENCE CONNORSJANUARY 20, 2015Having read the Georgia Institute of Technology Academic Honor code, I understand and accept my responsibility as a member of the Georgia Tech Community to uphold the Academic Honor Code at all times. In addition, I understand my options for reporting honor violations as detailed in the code.
Data and ResultsA.Separations of Pigments in Black Inka.Procedurei.A strip of filter paper about 4 inches long and 1 inch wide was cut and a pencil was used to draw a line about 1.5 cm from the bottom of the strip. ii.A small dot of black Expo Vis.à.vis wet erase marker ink was added to the center of the line. iii.10mL of water was added to a 250mL beaker and wrapped with a rubber band vertically to allow the filter paper a place to hang. iv.The bottom of the paper strip was immersed into the water without touching the bottom of the beaker and keeping the marker spot out of the water. The beaker was covered with plastic wrapv.The paper sat in the water until the water had risen close to the top without reaching it. Each pigment was circled, observations recorded, and the Rfwas calculated. vi.This same procedure (steps 1-5) was repeated an additional 2 times; however, different mobile phases were used. The first solvent was 5mL of acetone in combination with 10mL of water, and the second was 5mL of ethanol with 10mL of water. b.Data tablesRf=distancetraveleddistancethe mobile phasetraveledi.Table 1. Chromatography of black Expo Vis-à-vis marker in 10mL of water1.Total distance solvent moved: 7.1cmii.Table 2. Chromatography of black Expo Vis-à-vis marker in 10mL of water and 5mL of acetone1.Total distance solvent moved 6.8cm
iii.Table 3. Chromatography of black Expo Vis-à-vis marker in 10mL of water and 5mL of ethanol1.Total distance solvent moved 6.5cm