geneticslabreport - BIO206 Genetics Dr. Kirchner...

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BIO206 Genetics Dr. Kirchner Transformation of the Ade1 - Genotype in Yeast INTRODUCTION. Adenine is purine which has an important role in biochemistry most importantly in cellular respiration as it makes up adenine triphosphate an important chemical in energy. Adenine is derived from the nucleoside inosine monophosphate (IMP), which is synthesized on a pre-existing ribose through a complex pathway using atoms from the amino acids glycine, glutamine, and aspartic acid, as well as formate ions transferred from the coenzyme tetrahydrofolate. Throughout this lab we were trying to find genes required to allow a cell to make adenine. We did this first by mating the different genotypes of adenine mutants. Mutants are an organism with any change in their genotype. In this lab we want to find complimentary gene to the HA1 sequence. To find the Ade 1 - genotype in yeast cells geneticists must first start at the beginning with mating the cells. In mating the cells we found there was either complementation meaning that ade - strains mated to each other. When this happens it is determined whether the mutations that cause ade - phenotype have in the same order different genes, which may sort the mutants. If the yeast cells fail to complement each other then two strains with a mutations in the same gene, mating them together and generating a diploid would result in a
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geneticslabreport - BIO206 Genetics Dr. Kirchner...

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