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Unformatted text preview: Cleavage produces cohesive ends/staggered ends/ “sticky ends” 2 nd- Sticky ends officially hybridize with the foreign DNA that was fragmented by the same restriction enzyme. The hybridizing gets sealed by DNA Ligase- forming phosphodiester bonds 3 rd- by Transformation , plasmids are incorporated in bacterial host cells Each cell has different plasmids b/c each plasmid has different/unique DNA That is because during Transformation, the repair system makes sure that there are no nucleotide sequence differences of the donor/recipient DNA; therefore some cells get repaired and keep the ‘ampicillin resistance’, while others get rid of the ‘amipicillin resistance’. Transformation Refresh ….lol http://highered.mcgraw-hill.com/sites/0072556781/student_view0/chapter13/animation_quiz_1.html...
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This note was uploaded on 04/18/2008 for the course MICRO BIO 132 taught by Professor Ray during the Spring '08 term at Long Island U..
- Spring '08