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Lab 2- Microbio.docx - Microscopy, smears, and simple...

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Microscopy, smears, and simple stainsWatch this video tutorial on using a microscope (YouTube tutorial) and correct the underlinederrors in this protocol for how to use a microscope1.Clean ocular eye piece and objectives with lens paper. This paper won’t scratch thelenses.2.Turn on the microscope.3.Adjust ocular eyepiece to match your interpupillary distance. When looking through theeyepiece you should see oneimage(s).4.Lower the stage with the coursefocus adjustment knob.5.Place the slide onto the stage. Notice how it locks into place.6.Turn to the 10xobjective.7.Use the coarse focus adjustment knob to raise the stage.8.While viewing the slide, use the coarse focus to lower the stage until you see the image.9.Use the fine focus adjustment know to make the image clear.10. To see the slide at a higher magnification, turn to the 40x. Use only the fine focus tomake the image clear.11. To see the slide at the highest magnification, turn in between the 40x and 100x and add10 drop(s) of immersion oil onto the slide. Carefully slide the 4xlens into position. Useonly the fine focus to make the image clear.

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Term
Fall
Professor
N/A
Tags
Eyepiece, Marco Hernandez

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