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17 Proteome - BIO2045 Molecular Biology and Genome Analysis...

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BIO2045 Molecular Biology and Genome Analysis The proteome
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The proteome Single protein analysis methods Proteome analysis • 2D-gels • MALDI-TOF
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The proteome This lecture aims to: Outline analysis of the proteome
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The proteome After completing the learning activities associated with this lecture you should be able to: • Describe and appraise, with examples, methods for analysis of the proteome.
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During the lecture you should: • Listen Take notes as appropriate for your revision Attempt to answer questions Ask if you are puzzled After the lecture you should: Check you understand your notes Do required reading The proteome
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Genome Complete set of genes of an organism or its organelles Transcriptome Complete set of mRNA molecules present in a cell, tissue or organ Proteome Complete set of protein molecules present in a cell, tissue or organ Metabolome Complete set of metabolites (low M.W.) in a cell, tissue or organ
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Information content Genome Transcriptome Proteome Metabolome Context-dependent : levels change with physiology, development or pathology Context-independent
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Genome Complete set of genes of an organism or its organelles Transcriptome Complete set of mRNA molecules present in a cell, tissue or organ Proteome Complete set of protein molecules present in a cell, tissue or organ Metabolome Complete set of metabolites (low M.W.) in a cell, tissue or organ
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