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18 Metabalome - BIO2045 Molecular Biology and Genome...

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BIO2045 Molecular Biology and Genome Analysis Proteome interactions The metabolome Reverse genetics
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Proteome interactions The metabolome Reverse genetics Protein interactions Yeast two-hybrid analysis • TAP Metabolomics Reverse genetics Gene replacement Gene silencing
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Proteome interactions The metabolome Reverse genetics This lecture aims to: Describe methods for reverse genetics and for analysis of protein-protein interactions. Introduce metabolomics.
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Proteome interactions The metabolome Reverse genetics After completing the learning activities associated with this lecture you should be able to: • Describe reverse genetics methods • Describe methods for direct analysis of protein-protein interactions • Outline metabolomics
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During the lecture you should: • Listen Take notes as appropriate for your revision Attempt to answer questions Ask if you are puzzled After the lecture you should: Check you understand your notes Do required reading Proteome interactions The metabolome Reverse genetics
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Genome Complete set of genes of an organism or its organelles Transcriptome Complete set of mRNA molecules present in a cell, tissue or organ Proteome Complete set of protein molecules present in a cell, tissue or organ Metabolome Complete set of metabolites (low M.W.) in a cell, tissue or organ
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Information content Genome Transcriptome Proteome Metabolome Context-dependent : levels change with physiology, development or pathology Context-independent
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Genome Complete set of genes of an organism or its organelles Transcriptome Complete set of mRNA molecules present in a cell, tissue or organ Proteome Complete set of protein molecules present in a cell, tissue or organ Metabolome Complete set of metabolites (low M.W.) in a cell, tissue or organ
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DNA mRNAs Proteins Genotype + environment barb4right Phenotype
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