18 Metabalome - BIO2045 Molecular Biology and Genome Analysis Proteome interactions The metabolome Reverse genetics Proteome interactions The

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Unformatted text preview: BIO2045 Molecular Biology and Genome Analysis Proteome interactions The metabolome Reverse genetics Proteome interactions The metabolome Reverse genetics Protein interactions • Yeast two-hybrid analysis • TAP Metabolomics Reverse genetics • Gene replacement • Gene silencing Proteome interactions The metabolome Reverse genetics This lecture aims to: • Describe methods for reverse genetics and for analysis of protein-protein interactions. • Introduce metabolomics. Proteome interactions The metabolome Reverse genetics After completing the learning activities associated with this lecture you should be able to: • Describe reverse genetics methods • Describe methods for direct analysis of protein-protein interactions • Outline metabolomics During the lecture you should: • Listen • Take notes as appropriate for your revision • Attempt to answer questions • Ask if you are puzzled After the lecture you should: • Check you understand your notes • Do required reading Proteome interactions The metabolome Reverse genetics Genome Complete set of genes of an organism or its organelles Transcriptome Complete set of mRNA molecules present in a cell, tissue or organ Proteome Complete set of protein molecules present in a cell, tissue or organ Metabolome Complete set of metabolites (low M.W.) in a cell, tissue or organ Information content Genome Transcriptome Proteome Metabolome Context-dependent : levels change with physiology, development or pathology Context-independent Genome Complete set of genes of an organism or its organelles Transcriptome Complete set of mRNA molecules present in a cell, tissue or organ Proteome Complete set of protein molecules present in a cell, tissue or organ Metabolome Complete set of metabolites (low M.W.) in a cell, tissue or organ DNA mRNAs Proteins Genotype + environment b Phenotype Summary...
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This note was uploaded on 02/21/2008 for the course BIOLOGY 430 taught by Professor Aves during the Spring '06 term at University Of Southern Mississippi .

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18 Metabalome - BIO2045 Molecular Biology and Genome Analysis Proteome interactions The metabolome Reverse genetics Proteome interactions The

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