16 Transcriptome - BIO2045 Molecular Biology and Genome...

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BIO2045 Molecular Biology and Genome Analysis The transcriptome
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The transcriptome This lecture aims to: Outline analysis of the transcriptome
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The transcriptome Omes Gene expression analysis • Northern blot Transcriptome analysis • SAGE • Microarrays
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The transcriptome After completing the learning activities associated with this lecture you should be able to: • Describe and appraise, with examples, methods for analysis of gene expression at both the single- gene and genomic levels
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During the lecture you should: • Listen • Take notes as appropriate for your revision • Attempt to answer questions • Ask if you are puzzled After the lecture you should: • Check you understand your notes • Do required reading The transcriptome
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Definitions Genome Complete set of genes of an organism or its organelles Transcriptome Complete set of mRNA molecules present in a cell, tissue or organ Proteome Complete set of protein molecules present in a cell, tissue or organ Metabolome Complete set of metabolites (low M.W.) in a cell, tissue or organ
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Information content Genome Transcriptome Proteome Metabolome Context-dependent : levels change with physiology, development or pathology Context-independent
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Methods of analysis of the transcriptome Northern analysis SAGE Hybridisation arrays multiple genes - single-gene
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Northern blots Indicate presence and abundance of
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This note was uploaded on 02/21/2008 for the course BIOLOGY 430 taught by Professor Aves during the Spring '06 term at University Of Southern Mississippi .

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16 Transcriptome - BIO2045 Molecular Biology and Genome...

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