Catalase Enzyme Lab revised - Catalase Enzyme Lab...

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Catalase Enzyme Lab Background information Liver and other living tissues contain the enzyme catalase. This enzyme breaks down hydrogen peroxide, which is a harmful by-product of  the process of cellular respiration if it builds up in concentration in the cells. If we use potato or other tissue containing this enzyme, we can  use this to measure the relative influence of varying several different factors on the activity of enzymes in living tissue.  In order to obtain energy and building blocks from food, the digestive system must break down proteins, fats and carbohydrates. In this  process, specific enzymes catalyze hydrolysis reactions in which food polymers are broken up into monomers. In this lab, you will perform  reactions involved in digestion of carbohydrates, lipids, and proteins and observe the results of these reactions.  INTRODUCTION:  What would happen to your cells if they made a poisonous chemical? You might think that they would die. In fact,  your cells are always making poisonous chemicals. They do not die because your cells use enzymes to break down these poisonous chemicals  into harmless substances. Enzymes are proteins that speed up the rate of reactions that would otherwise happen more slowly. The enzyme is  not altered by the reaction. You have hundreds of different enzymes in each of your cells.  Each of these enzymes is responsible for one particular reaction that occurs in the cell. In this lab, you will study an enzyme that is found in  the cells of many living tissues. The name of the enzyme is catalase (KAT-uh-LAYSS); it speeds up a reaction which breaks down hydrogen  peroxide, a toxic chemical, into 2 harmless substances--water and oxygen. 
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  • Summer '13
  • Pereira
  • Test Tube, Catalase

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