Chapter 7 - CHAPTER 7 CONROL OF MICROBIAL GROWTH...

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Unformatted text preview: CHAPTER 7 CONROL OF MICROBIAL GROWTH Terminology of Microbial Control Sepsis Asepsis Aseptic techniques Sterilization Commercial Sterilization Sanitization Antiseptic vs Disinfectant Biocide or Germicide Biostatic Degerming Factors that influence the effectiveness of anti- microbial procedures: 1. microbial characteristics 2. number of microbes Decimal reduction time (D value) 3. environmental influences 4. time of exposure 1. Heat (moist & dry) 2. Filtration 3. Low temperature 4. High pressure 5. Desiccation 6. Osmotic pressure 7. Radiation Physical Methods of Microbial Control 1. Moist Heat Boiling kills most vegetative forms in 10 min Autoclave steam under pressure Sterilization occurs at 15 psi, 121 O C for 15 min Pasteurization Some heat resistant bacteria survive higher temp, shorter exposure time 1. Dry heat Incineration Flaming dry heat sterilization Requires higher temp and longer exposure 170C for 2 hours is equivalent to autoclaving 3. Low temperatures Slow freezing causes formation of ice crystals...
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Chapter 7 - CHAPTER 7 CONROL OF MICROBIAL GROWTH...

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