Chapter 7 - CHAPTER 7 CONROL OF MICROBIAL GROWTH...

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CHAPTER 7 CONROL OF MICROBIAL GROWTH
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Terminology of Microbial Control Sepsis Asepsis Aseptic techniques Sterilization Commercial Sterilization Sanitization
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Antiseptic vs Disinfectant Biocide or Germicide Biostatic Degerming
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Factors that influence the effectiveness of anti- microbial procedures: 1. microbial characteristics 2. number of microbes Decimal reduction time (D value) 3. environmental influences 4. time of exposure
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1. Heat (moist & dry) 2. Filtration 3. Low temperature 4. High pressure 5. Desiccation 6. Osmotic pressure 7. Radiation Physical Methods of Microbial Control
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1. Moist Heat Boiling kills most vegetative forms in 10 min Autoclave – steam under pressure Sterilization occurs at 15 psi, 121 O C for 15 min Pasteurization Some heat resistant bacteria survive higher temp, shorter exposure time 1. Dry heat Incineration Flaming dry heat sterilization Requires higher temp and longer exposure 170°C for 2 hours is equivalent to autoclaving
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3. Low temperatures Slow freezing causes formation of ice crystals
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