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Chapter 7 - CHAPTER 7 CONROL OF MICROBIAL GROWTH...

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Unformatted text preview: CHAPTER 7 CONROL OF MICROBIAL GROWTH Terminology of Microbial Control • Sepsis • Asepsis – Aseptic techniques • Sterilization • Commercial Sterilization • Sanitization • Antiseptic vs Disinfectant – Biocide or Germicide – Biostatic • Degerming • Factors that influence the effectiveness of anti- microbial procedures: 1. microbial characteristics 2. number of microbes – Decimal reduction time (D value) 3. environmental influences 4. time of exposure 1. Heat (moist & dry) 2. Filtration 3. Low temperature 4. High pressure 5. Desiccation 6. Osmotic pressure 7. Radiation Physical Methods of Microbial Control 1. Moist Heat – Boiling kills most vegetative forms in 10 min – Autoclave – steam under pressure – Sterilization occurs at 15 psi, 121 O C for 15 min – Pasteurization • Some heat resistant bacteria survive – higher temp, shorter exposure time 1. Dry heat • Incineration • Flaming • dry heat sterilization • Requires higher temp and longer exposure • 170°C for 2 hours is equivalent to autoclaving 3. Low temperatures – Slow freezing causes formation of ice crystals...
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Chapter 7 - CHAPTER 7 CONROL OF MICROBIAL GROWTH...

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