project13cola - Project 13 Analysis of Colas NAME Chemistry...

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Project 13: Analysis of Colas NAME Chemistry 101, Section 013 Instructor: NAME April 2, 2007 “My signature indicates that this document represents my own work. Outside of shared data, the information, thoughts and ideas are my own except as indicated in the references.” _____________________________________________________
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This experiment was performed to determine the phosphate concentration of different colas, for the purpose of making a competing cola with the right amount of phosphate in the ingredients. Important information derived was the proper use of the spectrophotometer instrument in a practice experiment using colored water and KMnO 4 solution. Knowledge of the relationship between absorbance, % transmittance, and wavelength was gained in this part of the experiment. Secondly, dilutions were made to fit a range of % transmittances so the points could be plotted on a line of best fit. Beer’s Law was used to obtain an equation which related the absorbance and concentration. A sample of .00101M KH 2 PO 4 solution was used to determine an equation for phosphate concentration that could be used for the colas of unknown phosphate content. Finally, dilutions of the colas were made and graphed using the Beer’s Law equation for KH 2 PO 4 to determine their phosphate concentrations. The colas used in this experiment were Coke, Dr. Pepper, and Pepsi. Introduction The purpose of this project is to determine the phosphate content of different colas in order to analyze the products’ major ingredient. To analyze the phosphate content, a spectrophotometer will be used. The spectrophotometer will give the absorbance and % transmittance of the cola samples. This information will then be used with the Beer’s Law to graph the results. This helps correlate the relationship between the light absorbed with the concentration of the sample in solution. It is important to learn how the spectrophotometer works before starting the experiment. This helps decrease errors in calculations if you know how to use the instrument properly. In order to acquire the proper data for this experiment, some practice samples will be performed and graphed to understand how the process will work. This is important to understand how the spectrophotometer works and how to graph and calculate results so you can transfer that knowledge to the next level of the experiment. The next step is to acquire the different colas that will be tested and run the samples through the spectrophotometer. The results will then be graphed, using a guideline obtained by the practice experiment. This will then help you determine the phosphate concentrations of each cola. In this experiment, the phosphate concentrations derived were .0083 for Coke, .0042 for Dr. Pepper, and . 0066 for Pepsi.
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project13cola - Project 13 Analysis of Colas NAME Chemistry...

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