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Unformatted text preview: BME 101 Formal Lab Report Computational Nerve Propagation Frog Sciatic Nerve and Temperature Effects Frog Electrocardiogram Lab Performed by: Joel Gotkin Partner: Robert Buechler (Week 3,4,5) (Patrick Parish for Lab week 2) (Jen Wei for Comp Lab week 1) TA: Letitia Holden Wednesday 425-715 pm Project Due: April 11, 2006 I hereby declare that have abided by the Duke University Community Standard in completing this lab report. Abstract: The Hodgkin-Huxley model can be simulated by computer, and can produce values of a maximum compound action potential (CAP), refractory period, and conduction velocity of a given nerve. The sciatic nerve of a frog is a very economical choice to perform laboratory experiments to associate physical values with the assumed computer results for the same parameters, due to its accessibility, length and thickness, and polarity. Temperature changes do affect these parameters, and it has been shown that for warmer temperatures, refractory period will decrease, whereas the conduction velocity and maximum CAP will increase. An electrocardiogram can be used to gain insight into the anatomy of the heart of a frog, and it also carries the ability to determine the heart rate of the frog. It was found that the heart rate of the frog decreases when the temperature of the heart is lowered. Introduction: The frog sciatic nerve is often used in nerve conduction experiments, as it is easily accessible through simple dissection, and is the biggest simple nerve in the body. The sciatic nerve arises from the lower part of the sacral plexus, and enters the gluteal region by the greater sciatic foramen of the hip bone. Figure 1: Sciatic Nerve of a Frog (seen in between the prongs of the forceps) http://www.biopac.com/bslprolessons/a02/A02isloate.gif The sciatic nerve is one of the media that allow for messages to be transferred from the brain or heart, anywhere in the body, even places as distant as the leg. The length of the frogs sciatic nerve allows for messages to be transferred all the way to the leg, even below the knee. These nerves contain axons of thousands of individual neurons held together by tissue. The messages are depicted as self-propagating action potentials along the axon of each neuron. Action potentials can be seen in a nerve by the methods of electrical stimulation. When a pulse is delivered, the flow of ions during the simultaneous action potentials can be large enough to be detected by measuring the difference in voltage between two points on the nerve. This can be simply done using a traditional oscilloscope, and the difference is termed the Compound Action Potential (CAP). This is not to be confused with the stimulus artifact, which is separate and unrelated to the CAP....
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This note was uploaded on 04/18/2008 for the course BME 101 taught by Professor Bursac during the Spring '07 term at Duke.
- Spring '07