Conservation Bio Main Points 10-10-07

Conservation Bio Main Points 10-10-07 - Conservation...

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Conservation Genetics and Phylogenetics Chapter 11 Conservation Biology Main Points 10/10/07 DNA turns to mRNA to Protein The DNA codes for the machinery of a cell Electrophoresis: Separates both nucleic acids as well as proteins. Nucleic acids mobility is dependent on size. Protein mobility on size and especially electric charge. Semi-conservative replication: Complimentary, not identical new strand o A with T, C, with G o Must be made single stranded to duplicate Polymerase chain reaction or PCR o Require primers to find a specific piece of DNA you want to analyze o Primers are specific to a particular gene so only one gene is amplified o Heating makes single strands o Cooling allows them to base-pair match or anneal o Taq polymerase resistant to high temperature Typical mammalian genome is on the order of 3 million base pairs PCR and Conservation Genetics o Do not need to know any molecular genetics of the target organism; universal primers o
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This note was uploaded on 04/17/2008 for the course E&EB 115 taught by Professor Jeffreypowellmartawells during the Fall '07 term at Yale.

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Conservation Bio Main Points 10-10-07 - Conservation...

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