Lab 5 Memo

Lab 5 Memo - applied voltages of varying amplitudes and...

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BE310-Analysis of Physiological Systems Lab 5: Measurement of Response Properties in the Frog Gastrocnemius 11-14-2007
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Introduction: In this lab we dissected the leg of a frog, and removed the gastrocnemius muscle from its lower leg. Using an electrode controlled by computer we applied voltages of varying amplitude and frequency to the muscle. We then used a transducer to measure the muscle tension. For part two, we used a displacement transducer to measure how much the muscle contracted. Experimental Procedure: We started out by isolating the gastrocnemius muscle and removing. We hung the muscle from our apparatus and used a frog ringer solution to ensure that the muscle remained functional. We then mounted electrodes to the apparatus and made sure that the probes had adequate contact with the muscle. For part one of the experiment, we attached the force transducer to the muscle and
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Unformatted text preview: applied voltages of varying amplitudes and frequencies. We used LabScribe to gather the contractile data from the experiment. For part two, we disconnected the force transducer and replaced it with a displacement transducer. As in part one, we applied voltages of varying amplitudes and frequencies. We again used LabScribe to collect the data. Data/Discussion: Below is a screen shot of muscle summation induced in the muscle with a stimulation of 10 Hz with an amplitude of 1Volts. Figure 3 is a plot of velocity of muscle shortening as a function of loading force. Figure 3: Load vs. Velocity 0.5 1 1.5 2 2.5 3 3.5 100 200 300 400 500 600 700 Load (N) Velocity (V/s) Figure 4 below shows the contractile force generated as a function of stimulus voltage. Figure 4: Reaction to Impulse 1.46 1.48 1.5 1.52 1.54 1.56 1.58 1.6 1.62 0.2 0.4 0.6 0.8 1 1.2 1.4 1.6 Amplitude of Stimulus (V) Force (V)...
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Lab 5 Memo - applied voltages of varying amplitudes and...

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