CHapter 3 Quiz

CHapter 3 Quiz - 1. If one wanted a very precise...

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1. If one wanted a very precise determination of the mass of a protein, what would be the method  of choice?  a. Gel Filtration chromatography b. SDS polyacrylamide gel electrophoresis c. Mass spectrometry 2. A protein has been eluted from a DEAE-cellulose column by washing with a salt-containing  solution. Prior to assay, you need to remove the salt as it interferes with the activity to be  measured. Which of the following would NOT accomplish the removal of salt from your sample?  a. Passage through a carboxymethyl-cellulose column b. Passage through a gel filtration column c. Dialysis Correct. This type of chromatography would not be expected to remove salt from your sample.  Think of the basis for separation of various techniques.  3. SDS polyacrylamide electrophoresis could be used to do which of the following?  a. Determine the molecular weight of an oligomeric (multisubunit) protein b. Determine the molecular weights of subunits of an oligomeric protein c. Purify a monomeric protein serving as an enzyme in its active form Correct.  You would be able to determine the number of types of subunits and their molecular  weights. Without additional information, it will not be possible to use this method to determine the 
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CHapter 3 Quiz - 1. If one wanted a very precise...

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