Course Hero Logo

Finish Prot Funct Regul exam resources FAQs 2nd time.pdf -...

Course Hero uses AI to attempt to automatically extract content from documents to surface to you and others so you can study better, e.g., in search results, to enrich docs, and more. This preview shows page 1 - 12 out of 32 pages.

Proteins! Part TWO.five!Module 1Readingpg 121-145, 150-157PROTEINSRegulationWhereHowPhosphorylation vs. GTP binding
We can build them,we can determine their function,BUT how does the cell control them?
Feedback regulationcontrolsbiosynthetic pathwaysProducts from a LATERpart of the pathwaySTOP the processWhat would we call it if aproduct from the bottom part ofthe pathway, made MORE “A”?
Feedback regulationcontrolsbiosynthetic pathwaysProducts from a LATERpart of the pathwayAFFECT the processPositive = TURN ONNegative= TURN OFF+In addition to the active site,what must a protein have to be regulated?
Regulation sitesdeterminethe action of a protein (best example: enzymes)Likely a totally different binding siteRegulator causes aconformational(shape)changeConformational change can decrease OR increase aproteins activity/binding abilityProtein isallostericif it can have 2+ different shapesRegulation sites areA) IrreversibleB) ReversibleC) Non-existantD) Only in Plants
A chemical group can act as an inhibitor/activatorKinase:attaches phosphate groupProtein more negative, pulls positive aanear itPhosphorylationPhosphatase: removes phosphategroupProtein is more positive, pushespositive aa awayDephosphorylationDoes adding a phosphate mean a protein MUST be inactive?A) YesB)No
There can be more than one modificationMultiple TYPES of modificationsAcetylation (add acetyl)Addition of Fatty Acid (addFatty acidname-ation)Ubiquitination (add ubiquitin)Combinations of modificationsdetermineActivityLocationBinding partners
Sticking with the idea of conformational changes…A protein can literally WALK due to it’s binding siteConformational changes justchange shape becausethey’re reversibleMotor proteins: causemovement ( byconformational change) butIN A DIRECTION so it doesn’treverseSo what smallmolecule do youknow that can beadded that carriesEnergy AND is LIKELYNOT reversible?A) WaterB)ATPC)Fatty AcidD)Amino Acids
Sticking with the idea of conformationalchanges… A protein can literally WALK due to it’sbinding siteConformational changes justchange shape because they’rereversibleMotor proteins: causemovement ( by conformationalchange) but IN A DIRECTION soit doesn’t reverseTo watch it live….watch?v=xysUmcsN5DM
Guanosine Triphosphate (GTP) bindingproteins act like an ON/OFF switchesGTP binding proteins have the ability toself-hydrolyzeAdditional proteins can ALSO hydrolyze GTPIf the cell can regulate activity with concentrations, what areTWO different targets for regulating a GTP binding protein?

Upload your study docs or become a

Course Hero member to access this document

Upload your study docs or become a

Course Hero member to access this document

End of preview. Want to read all 32 pages?

Upload your study docs or become a

Course Hero member to access this document

Term
Fall
Professor
NoProfessor
Tags

Newly uploaded documents

Show More

  • Left Quote Icon

    Student Picture

  • Left Quote Icon

    Student Picture

  • Left Quote Icon

    Student Picture