Enzyme Lab - The Effects of Temperature and Concentration...

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The Effects of Temperature and Concentration on the Activity of the Enzyme Catalase found in Broccoli Lab T.A.: Ali McGregor Lionel Sims III Date of Lab: 09/16/07
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Abstract This lab report basically evaluates the function of enzymes. In particular catalase derived from broccoli (Brassica oleracea var. italica ). The procedure simply puts the solution of catalase into different conditions and tests its reaction time with a piece of filter paper and H2O2. The goal was to determine what would have the greatest effects on its reaction rates. My hypothesis was that the effectiveness would lower with the concentration, the data confirmed this by suggesting that increasing the concentration of catalase can increase rate of reaction, the reaction time almost went down almost by a factor of 4 with each increase in the enzyme’s concentration. A solution with an unknown concentration was tested against a standard curve, reached through graphing the rates of solutions with known rates. I also hypothesized that increased temperature would increase reaction rates. The data showed increased rates until the temperature of denaturizing was reached. It was also confirmed that the shape of the enzyme determines its effectiveness on its substrate. Introduction : The lab and all of its experiments are designed to analyze what conditions do and don’t affect catalytic processes of enzymes in their intended reactions (e.g. concentration). Because that is what an enzyme is for, it is a biological catalyst for chemical reactions that must take place inside animal and plant cells. Enzyme function relies on its ability to bind with the reactants (also called its substrate) on its active site, which is a specifically shaped part of the enzyme that makes it designated to only one chemical reaction. Enzymes are also proteins produced by the cell when they are needed, which is why some parts of the experiment delve into conditions that could also alter basic protein functions, such as temperature and chemical alteration (Campbell and Reece 2005). The enzyme I used in this lab was catalase. It is found in both plant and animal cells and its function is to break down H2O2 into water and oxygen.
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2 H2O2 2 H2O + O2. This reaction is necessary in order for cells to have Hydrogen peroxide homeostasis, (T. Yang and B. W. Poovaiah 2002) which provides safety from free radicals in a cell. The catalase we used came from broccoli (Brassica oleracea var. italica ). It was obtained by breaking the cell walls of the broccoli in a blender and mixing with a little bit of distilled water in order to render a useful sample of the catalase. This lab is useful for getting visual representation of biological processes and gaining a better understanding of what can or can’t have an affect on their occurrence. I believe that the reaction rates of the catalase enzyme will be faster in higher temperatures to a certain point, that it doesn’t become denatured, as would be the
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Enzyme Lab - The Effects of Temperature and Concentration...

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