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Unformatted text preview: Sheldon Pope Bio Lab section L19 November 1, 2007 Effect of pH on Enzymes. I ntroduction: Enzymes are very important in many cellular tasks and are also very important in the metabolic process. There are enzymes in your mouth that start the digestive process as soon as you ingest food. Enzymes are catalysts which lower the amount of energy needed to perform a reaction. So in short enzymes speed up certain reactions. Enzymes have extremely complex shape which allows them to bind with the reactant or substrate. Enzymes are used in almost every biochemical reaction within the cell. pH is an element which can affect the rate at which enzymes react. Molecules that affect enzymes are inhibitors or activators. Activators help with enzyme reactions while inhibitors slow them down, thus the name “inhibitors” and “activators”. Inhibitors interfere with enzymes either by filling the binding sites and competing with substrate molecules or now makes the enzyme unable to bind. The purpose of this lab is to observe several examples of enzyme activity, and analyzing what it means. Pectinase is an enzyme which is used to make juice from fruits. That is the first part of the lab, the second is to see what pH and temperature affect in the enzyme Catecholase. The final part of the lab is to design an experiment to measure the effects of pH or temperature on Catecholase enzyme. The purpose of enzymes will become evident in this lab. Materials & Methods: Part 1: A. Display of water saturated apple sauce and pectinase saturated apple sauce....
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This lab report was uploaded on 04/18/2008 for the course BIOLOGY 41 taught by Professor Albaker during the Fall '08 term at New Hampshire.
- Fall '08