bio lab report11 - Sheldon Pope (and Allison, Lam, and...

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Sheldon Pope (and Allison, Lam, and Eric) Biology 412 Lab Section L18 Bacteria and Protista Lab
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2-14-08 Introduction: E. coli is a prokaryotic bacterium that lives within many organisms including humans. Due to the fact that it lives within our digestive system E. coli has a symbiotic relationship with humans. Gram staining is a method to determine the difference between certain types of bacteria. Gram negative indicates that there are two cellular membranes while a Gram positive bacterium only has one membrane. Gram negative bacteria are typically pathogenic meaning they harm host cells. Antibiotics can be literally translated to things that are against bacteria. Antibiotics are often products of other bacteria. Bacteria compete in order to survive; therefore most bacteria secrete a type of inhibitor which blocks their competition from spreading. Protists are single or multi celled organisms. They are a diverse group of simple organisms. Some are animal-like, plant-like, or even fungi-like. The phylum rhizopoda contains organisms that move using extensions of their cytoplasm. The most commonly known rhizopoda is the amoeba. Phylum ciliophora contains single celled organism that are recognized by the small hair resembling cilia on the outside of the cell. Phylum zoomastigophora are recognized by their flagellum. The purpose of this lab is to figure out whether a few normal everyday things are antibiotic and to figure out whether other not so ordinary things are antibiotics. The bacteria which are being tested normally live on and within people. Materials and Methods: Our bacterium was E. coli; in this lab we used possible common antibiotics such as hot sauce, garlic oil and shampoo. We also used known antibiotics; these were neomycin,
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tetracycline, penicillin, and photorhabdus. More things needed for part one were three petri dishes, cotton on a stick, sterile paper circles, a permanent marker, and tape. The next part of the lab required Gram staining equipment; a flame, platinum loop, crystal violet stain, 95% ethanol
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This note was uploaded on 04/18/2008 for the course BIOLOGY 41 taught by Professor Albaker during the Spring '08 term at New Hampshire.

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bio lab report11 - Sheldon Pope (and Allison, Lam, and...

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