Chemical Aspects of Digestion Lab Report

Chemical Aspects of Digestion Lab Report - Clarke 1 1....

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Clarke 1 1. Analysis of parts A-E: a. In part A, the enzyme used was Pancreatic Lipase. Hydrolysis occurs in the duodenum. The optimum ph range is 7.1-8.2. “The substrate is triglyceride and the products are glycerol, fatty acids, and glycerides.” (Campbell and Reece 2005) The macromolecules tested were lipids. Phenol Red was used as a ph indicator test. If the lipids are digested then there will be a color change. If the solution is acidic then more triglycerides were digested because of an increase in fatty acids. If the lipids were acidic then the phenol red would turn the contents of the tube yellow. If the lipids were neutral then the contents of the tube would turn orange. If the lipids were alkaline then the contents of the tube would be red. If the contents of the tube had a ph of 10 or greater then it would turn pink. b. Triglyceride Digestion with Pancreatic Lipase Observations Results Tube 1 Orange + Tube 2 Reddish Orange + Tube 3 Pink - c. Tubes 1 and 2 worked because the enzymes were fully functional, nothing was present to inhibit them. Where as tube 3 was boiled, so that increased the rate at which the enzymes were going. When the test tube was incubated little to, no enzymes were present. d. In part B, the enzyme used was Pancreatic Proteases. The different types of pancreatic proteases include trypsin, chymotrypsin and carboxypeptidase. Hydrolysis occurs in the duodenum. The optimum ph range in 7.1- 8.2. The substrate is protein and the products are polypeptides and amino acids. The macromolecules tested were proteins. The time frame was an hour. The two tests used were the coagulation test and biuret test. The coagulation test tells whether the enzymes digested the protein. If flocculation was present after boiling the solution then the protein was digested. If the precipitate was chunky or a clump was present then the protein was not digested by the enzymes. The biuret test tells if polypeptides were present in the solution. If polypeptides were present then the solution would be a light violet to pinkish color. If proteins were present then the solution would turn a darker purple color. e. Protein Digestion with Pancreatic Proteases Test For Observations Coagulation Results Tube 4 None - Tube 5 Chunk Protein not digested by enzyme
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Clarke 2 Tube 6 Flocculation Protein was digested Protein Digestion with Pancreatic Proteases Biuret Observations Test Results Tube 4’ Blue - Tube 5’ Dark Purple Proteins present Tube 6’ Light Purple Polypeptides present f. In the coagulation test for test tube #4, no coagulation was present, this was the control. In test tube, #5 chunks were present but the enzyme did not digest the protein. The protein was not digested because it did not have an optimum ph, the ph was too acidic. Test tube #6 had flocculation present this means that the protein was digested. The proteins were digested because it had an optimum ph. In the Biuret test for test tube, #4’ had a negative result because there was no protein in it, this was the control. Test tube #5’ turned dark purple when
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This note was uploaded on 04/18/2008 for the course BIOL 126 taught by Professor Ropski during the Spring '08 term at Gannon.

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Chemical Aspects of Digestion Lab Report - Clarke 1 1....

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