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Unit 6 Animal Handling and Blood Collection

Unit 6 Animal Handling and Blood Collection -...

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Introduction -Animal Handling and Blood Collection Blood Sampling : Blood sampling is a very common procedure but it requires knowledge of an animal's anatomy and physiology. In order to sample blood, the body's primary defense, the skin, must be breached. Thus, aseptic technique should always be used. The process of collecting blood (handling) is stressful for the animal and every effort should be made to make the animal feel as secure as possible. Stress may produce physiological changes, which would need to be considered when experimental data are interpreted. In order to minimize stress, and thus reduce between-animals variation, animals should be handled carefully, calmly, and conditioned to the procedure (ie habituated to handling prior to blood collection procedures). Restraint, sedation, or anesthesia should always be considered and may be required to immobilize the animal for a procedure. Small laboratory animals are usually placed in restrainers that secure the conscious animal and simultaneously allow access to peripheral blood vessels (lateral tail veins in mice and rats; ear veins in rabbits). In some species (gerbils and guinea pigs), blood from peripheral vessels is difficult and other sites such as the heart or retro orbital sinus need to be used. In order to access these sites safely, sedation and /or anesthesia is required. Handling, restraint, anesthetics/sedatives, and injection techniques will be taught in the wet lab portion of the training program. Physiological Basis for Blood Collection The amount of blood that can be safely taken from an animal depends on the total blood volume, and the volume and rate at which blood is to be removed. Total Blood Volume The circulating blood volume of most species is between 6% and 8% of the body weight. Using 6% as the conservative estimate, the total blood volume of most species is 6 ml per 100gm; or 60 ml per kg (note that this is in gm and kg NOT lb.; for Imperial measures, it is 60 ml per 2.2 lb.)
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