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Chapter 6 Notes - Chapter six A tour of the cell Methods of...

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1000 g G = Force of gravity 150000g 100000g 20000g 10 min. 3 hrs. 60 min. 15 min . Pellet (nuclei) Pellet (mitoch-ondria) Pellet (membrame frag. Pellet Riboso-mes Plasma membrane Nuclear envelope Cytoplasm Nucleus Chapter six A tour of the cell Methods of cell study 1. Microscopy – makes cells and their structures visible a. Characteristics of microscopes - Magnification – ratio of image to actual size - Resolution – minimum distance at which two points are seen as separate b. Types of microscopes (figure 6.3 and 6.4, compare micrographs) Property Light microscope (LM) Electron microscope (EM) Source of image Beam of light focused by lenses Beam of electrons focused but magnets Max magnification Max resolution 1000 X 0.2 µm (1 µm = 10 -3 mm) 100,000 X 2 nm (1 nm = 10 -6 mm) Smallest object visible Bacteria Mitochondria DNA molecules Ribosomes, plasma membrane Disadvantages Can’t see many organelles Can’t study of living cells Transmission (T.E.M.) Scanning (S.E.M.) Internal (ultra) structure surface features 2. Cell fractionation
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