Genetics Final Study Guide - Genetics Final Study Guide Transduction is a side effect of bacteriophage-LYSIS infection Transduction happens when a newly

Genetics Final Study Guide - Genetics Final Study Guide...

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Genetics Final Study GuideTransductionis a side effect of bacteriophage---LYSISinfection Transduction happens when a newly assembling virus accidentally bundles up some bacteria DNA into its capsid! Transformationis the integration of DNA fragments from the environment Conjugationis governed by the F-factor F-+ x F- = All F+F+ x F+=All F+F- x F-=No Mating OccursTwo main differences between F+ and Hfr strains: In Hfr, the F factor is integrated into the chromosome.During conjugation, the Hfr’s F factor is usually NOT completely transferred to the recipient cell. next generation cells are all F+ no change Calculting Titer:Titer = (# plaques / mL) x (dilution factor) = (2470)(10^6)Multiplicity of Infection (MOI) The average number of phage particles that infect a single bacterial cell in a specific experimentControl MOI by controlling ratio of phage to bacterial cells “Low MOI” (<1 phage/cell) “High MOI” (>2 phage/cell) Culturing lytic phage in the lab 1. Mix together bacteria and phage 2. Grow bacteria and phage
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3. Wait for phage to lyse bacteria
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