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Chromatography - Chromatography Lab Section 2119 Course...

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Chromatography September 19, 2007 Lab Section 2119 Course Number 1391 Yanyan Huang Amanda Quella
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Purpose: In this experiment the purpose is to separate pure substances from heterozygous substances by using chromatography. Data and Observations: Column Chromatography of Leaf Pigments: Test Tube *Color Intensity Personal Observations on Color 1 10 Brightest Green 2 6 Light, almost lime green 3 1 Clear, with barely a hint of green 4 0 No Color 5 0 No Color 6 0 No Color *Color Intensity is based on a scale from 1 to 10, with 10 being the most intense. Solvent Test Tube Number of Drops Time Speed (drops/second) Hexane 1 20 30 sec 0.67 2 14 30 sec 0.47 3 15 30 sec 0.50 Acetone 4 15 30 sec 0.50 5 18 30 sec 0.60 6 11 30 sec 0.37 Color Intensity of Test Tubes 0 2 4 6 8 10 12 0 1 2 3 4 5 6 7 Test Tube Color Intensity *Color Intensity is based on a scale from 1 to 10, with 10 being the most intense.
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Paper Chromatography: Observations of Color: Dye Observations Yellow Industrial light pink on the bottom and bright, intense yellow on top Green
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