Bio Test 2 Practice - Biology 131 Examination 2 March 2 2004 You may wish to remove this page for easy reference If you do be careful to leave the

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Unformatted text preview: Biology 131 Examination 2 March 2, 2004 You may wish to remove this page for easy reference. If you do, be careful to leave the other pages stapled together. f t ,for a monovalent cation, X. For a diavalent ion, the above equations must be divided by 2 (z = 2), thus VCa = 29 mV log (Gag/Cat). For a monovalent anion such as Cl', 2 = -1, so V0 2 -58 HIV 10g (Clo/Ch). V... = V.- - V0 1: = 105 coulombsfmole (Rn?) = 25 mV nv=vi—‘Pz “HP—H A‘P=lfi—Pu)“(ns-na) antm 1’1 = RTZ C: a 25 2 Cr where C; refers to the concentration ofthe i different i "101 t solutes in the solution. L = #61): “MD: flDD; in 3, 2 and 1 dimensions, respectively. Thus, t increases with L2, so t = LZHGD, L2H4D, 1.2320. The pressure generated by the meniscus of water: P = -2T!rc, where T is the surface tension ofwater (7 x 10'8 MPaom=70 x 10'3 me) and rc is the radius ofcurvature ofthe meniscus. 1 Pa =1joulex’m3; 1 atm = 0.1 MPa = 760 mmHg m 10 m H30 Constants, Algebraic Relationships, and Conversion Factors log 10 = 1; log 2 z 0.3; log 3 z 0.5; log 5 a: 0.7"; log 100 = 2; log 1000=3, etc. log (NB) = »log(BIA) = log A — log B log (A-B) = log A + log B; thus log 50 = log 10 + log 5 =1+ 0.? =1] log(10") = x Avogadro’s number = 6x10” 114m :10} mm =104 cm 1 L=103 mL =103 cm} As prefixes to units, c = 102, m = 103, n: 10'“, n = 10"), K = 103 For a sphere, Volume = 4nr3x’3, Area = 4mg, In Bio 131, n = 3. Speed of light in vacuum, c = 3 x 108 m/s; energy ofa photon. E = hcfl, h = 6.610'34jou1e o s Biology 131, Examination‘l, Spring, 2004 Name (Printed) Signature Student Number Lab Secti0n (write “none if not taking the lab) Enter your name and student number in the space above and also on the following pages. Answer the questions in the space provided; do not write on the back. Remember that your answers will be graded by a human being, not a machine, so legible writing is essential. If your handwriting is poor, print your answers. You must write in ink if you want to have the possibility of a re-grade. l. (10) A. (5) Suppose that the concentration of Ca2+ outside a cell is 1 mM and the membrane potential of the cell is — 58 mV. If Ca2+ were in equilibrium across the plasma membrane, what would be the concentration of Ca2+ in the cytosol of the cell? This is a Nernst equation problem. IF (‘33)); A3“ 2 - 55’ vz 5.? ———fl-'*““’ m 9‘ i9?— CM Ivam __ : D _,_._._. 1 l ah Cox] Cq‘, ’L B. (2) What is the typical concentration of Ca2+ in the cytosol ofcells? IOOnm C. (3) What happens to the Ca2+ concentration in the cytosol ifCa2+ channels in the plasma membraneopen? We have discussed two specific, immediate consequences of this change in Cal)“. Brie fly and specifically identify one of them. That is, say what cell . . . . . . 1-.— . . IS involved and what the immediate, smgle consequence ofthe change in Ca‘ IS in that case. he? Name (Printed) 2. (10) A. Water efflux from the guard cell G. Opening ofCah channels and increase in intracellular Caz? . Membrane depolarization (Vn1 becomes more ositive) I. Membrane hyperpolarization (Vm becomes more neative) B. Activation of receptor by ABA C. Opening ofCl' channels and Cl' efflux D. Activation of H+-ATPase J. Secondary transport of Cl‘ into guard cell E. Water influx into the uard cell. K. K+ efflux F. Activation ofblue liht recetor L. K.+ influx A. (5) 1. (2 points) In the blank below, write the letters of the first and last events in the opening of a stoma in a leaf. Choose the letters from the grid above. You must get both right to get any points ' First event E Last event E 2. (3 points) In the blank below, write FOUR letters of the events that occur between the first and last event that you identified in 1 above. You must get all four right to get any points, and you must not write more than four letters. The order of the letters is not considered. B. (5) 1. (2 points) In the blank below, write the letters of the first and last events in the closing of a stoma in a leaf. Choose the letters from the grid above. You must get both right to get any points First event Last event A 2. (3 points) In the blank below, write FOUR letters ofthe events that occur between the first and last event that you identified in 1 above. You must get all four right to get any points, and you must not write more than four letters. The order ofthe letters is not considered. Intermediate events (fourletters): C, GI H“, K or Name (Printed) 3. (5) Below are shown chemical struct s of six plant hormones. Among them are auxin (indole acetic and abscisic ABA). Circle auxin and ABA. You don’t need to distinguish be ween auxin and ABA. You must get both right to get any points. Hx—CHl fl HN—CHg—CH :c/CH" 0 _ CPLOH . .\. _ N/ . -/ 'N r l ‘> ‘> “- x N N H H H H \C_ / HO / _C\ H H HO 4(5) What is the experimental evidence for the polar transport of auxin in seedling hypocotyls‘? You are not asked here to explain the cellular basis of polar transport. 'The use of a simple, clearly labeled diagram may save words. ’ ’1‘ “we WM aux‘M new“L w X‘s..- w- «Wm ac auxlchWlflflA—Q he? Name (Printed) 5. (10) A. (5) Gibberellin (GA) is made by the developing plant embryo and acts to cause starch breakdown in seeds. One aspect of this process involves Ca2+. Identify the cells that are involved, and use a diagram and a few words to show how GA is connected to Ca2+ and starch breakdown. 09000000 B. (5) The GA pathway described in A. also includes gene transcription. Explain briefly the role of gene transcription in starch breakdown. Again, a diagram will help save words. KW Name (Printed) 6. (10) In a clearly labeled diagram, show the basis for the polar transport of auxin in a hypocotyl cell. Be sure to show the important physiological difference between the cell wall and the cytoplasm, and explicitly show the structural polarity that is required for polar transport. Label the apical-basal polarity of the cell. £19}ch 1219-9“ l 305A 7. (5) A. (3) What is the difference between the wave properties of blue light versus red l... ..l....... WW. Wu a... .9 B. (2) Does a photon of blue light have more or less energy than a photon of red light? Justify your answer briefly by reference to the relevant equation. Name (Printed) 8. (20) A. (5) What process leads to the formation of the megaspore from the megasporocyte and how many functional megaspores are produce from a single megasporocyte? This questiou can be answered with one word and one number. '14de I B. (4) What critical, unique event occurs during the process in part A, and what is the function of that event? ‘ ‘ Mn N. 4-01 “Have MUN“? o? W W 0‘1 C. (6). On the diagram below, label the indicated cells and give their ploidy (number of complete set of chromosomes). CLO—11 er Plead} thitr‘itXmLQ 1A) in) Sangiéfck l‘fL) D. (5) Briefly describe the process of fertilization in angi05pen'ns, making explicit reference to the components that you have identified in 13 above, and the contribution from the male gametophyte. You are not asked here to talk about the formation of the male gametophyte or how it gets to ovary. Just focus on fertilization. (Alng im emu-HM. nae sham—7:11) r MW. P m / MAQSL/kqu 6-141-0- W W 5W6 M "ma Mwwfmm’tkflew willie wire-Q e Name (Printed) 9. (10) Shown below on the left are the absorbence spectra for the two forms of the phytochrome molecule. The x-axis of this graph shows the fraction of the light that is absorbed by a suspension of the molecules as a function of the wavelength of the NW right are the two forms of the phytochrome molecule. ~ Thioether anage A‘DSOI’bGHCf‘ HUD Soc boo '4 O Wauclt’u3fl4 (nun; 8.0 A. (2) Which absorbance spectrum, 1 or 2, is for P”? l Which is for Pr? CL B. (2) In the representation ofPr, circle the part of the molecule that absorbs light. Circle the protein part of the P;—,_ C. (6) Suppose that lettuce seeds that have been stored in the dark are exposed to a pulse oflight of wavelength 610 nm. What form will phytochromc be in, P, or P1}? PE . Will the seeds germinate? %é4 Suppose the same seeds are then exposed to a pulse of 7'30 nm light. What form will phytochrome be in? FF Will the seeds germinate? an Suppose the same seeds are then exposed to sunlight. What form will phytochrome be in? P: 2: P451: Will the seeds germinate? w S Kg? 10. (5) Ifthe total solute concentration in the cytosol ofa plant cell is 200 mM and the total solute concentration outside the cell is 100 mM, what will be the turgor pressure of the cell? Express your answer both in atmospheres and pascals. K 7- TV; “Pi—re (9“ 4—56 :0) WE”! o = RT (C; 4.0) 2 35‘ (20am — room“) L, as“ . ‘00. [0—3 Ml __._ 1.5 arm :o.asr~1Pq Moi L Name (Printed) .4. U F-F‘b I 11. (5) The generation of negative pressure sufficient to raise water to the tops of tall trees depends on having ain'water interfaces that can force the surface of water into shapes with small radii of curvatures. This occurs on the cell walls of cells within the leaves. What must the radius of curvature of water he in order to generate pressure required to just raise water to a height of 100 m? :. —- £3: Prmw Matuircd +0 "(Mise- LIV-DAM r ~11“ ,, . ¥xro“9MPa'm - Halo—Em. H: —- fl -_—-—-________,_._.—.._.—-—-' —. c- ? ..—- (mpg 12. (5) Plants require nitrogen from the soil. In legumes, the roots become infected with Rhizobium bacteria, which convert atmOSpheric nitrogen to a useable form. What is the chemical form of atmospheric nitrogen? N2. To what chemical form do the bacteria convert nitrogen? N H3 . Other bacteria in the soil convert the nitrogen into a form that is non~toxic to plants and can be taken up by the plants. What is that chemical form? Qo; . 1‘ To what chemical form do plants convert nitrogen in the chloroplasts? N”; E'- N H's} What is the major direct (not final) product that plants synthesize from the form of nitrogen mentioned above? Can-«1WD- acflia. . ...
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This test prep was uploaded on 04/18/2008 for the course BIOL 131 taught by Professor Robinson during the Spring '08 term at Purdue University-West Lafayette.

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Bio Test 2 Practice - Biology 131 Examination 2 March 2 2004 You may wish to remove this page for easy reference If you do be careful to leave the

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