Bio test 3 practice 5

Bio test 3 practice 5 - Exam 3-06 Biology 131 Examination 3...

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Unformatted text preview: Exam 3-06 Biology 131 Examination 3 April 4, 2006 You may wish to remove pages 1 and 2 for easy reference. If you do, be careful to leave the other pages stapled together. N0 CALCULATORS! J = -DAC/Ax; J = -PAC zfl [362]. F2 [962].- Vx ,for a monovalent cation, X. X o X Vx = 25mV ln X = 58mVlog For a diavalent ion, the above equations must be divided by 2 (z=2), thus Vca = 29 mV log (Cao/Cai). For a monovalent anion such as Cl", 2 = -1, so Va: -58 mV log (Clo/C11). F = 105 coulomb/mol (RT/F) = 25 mV Vm = _ V0 ‘I’ = ‘PP +‘PS(= P—H) A9? = ‘1’2 —‘I’1=(‘I’P2 —‘I’Pl)—(‘P“ 41’s,), at equilibrium, A‘P = 0. W3 = 421,2le =_2'5.103 Joules 2C" = _2.5 L MPa EC” "1018 n ma] n where Cn refers to the concentration of the n different solutes in the solution. L = /6Dt , (4Dt’ , IZDt in 3, 2 and 1 dimensions, respectively. Thus, t increases with L2, so t = L2/6D, L2/4D, L2/2D. The pressure generated by the meniscus of water: P = -2T/rc, where T is the surface tension of water (7 x 10‘8 MPa-m) and rc is the radius of curvature of the meniscus. 1 Pa = 1 joule/m3; 1 atm = 0.1 MPa = 760 mmHg z 10 m H20. Constants, Algebraic Relationships, and Conversion Factors log 1=0; log 10 = 1; log 2 z 0.3; log 3 z 0.5; log 5 z 0.7; log 100 = 2; log 1000=3, etc. log (A/B) = -log(B/A) = log A — log B log (AIB) = log A + log B; thus log 50 = log 10 + log 5 =1 + 0.7 = 1.7 log(10x) = x Avogadro’s number = 6x1023 1pm :103 mm =10'4 cm 1 L=103mL=103 cm3 As prefixes to units, 0 = 102, m = 103, u = 106, n = 109, K = 103 For a sphere, Volume = 41rr3/3, Area = 4m2, In Bio 131, 1r = 3. Speed of light in vacuum, c = 3 x 108 m/s; energy of a quantum, E = hc/k, h = 6.6 x 10'34joule s Exam 3-06 Nucleotide Bases. NH2 0 NH2 0 o </N I \N (NIKNH N/j HN HN I /l N A 0% 0* 0* Adenine (A) Guanine (G) CytOSine (C) Thymine (T) Uracil (U) HO HO SECOND POSITION THIRD posmow (3' END) FIRST POSITION (5‘ END) U C A Glycm (Ga-x Alanine (Ala) A Valina (Val) V Leucine (Lou) lsoleucine (lle) Glutamine ¢° .. .. H _ (Glu) CH, c ' c‘nu, Tyrosme CH CH (TY') / = \ —CH *‘C C—‘OH Y ' ‘CH—ClV \ —CH;—C=CH Hlslldine ll,“ A (Hi5) \I H CH Lysim (“grew—CH.-carom-Km, K Arginina (N9) /NH, —CH.—CH,-—CH, —NH,—C‘ . NH. \ / °\_/N\ Cysteine _CHI_5H (Cvs) C. Methionine __ __ _ _ (Me!) CH, CH. 5 CH, Aspartic acid I ¢° — H —C (Asnl ‘3 ’ \o— D Glulalnic acid _CH __CH _c,¢° (Glu) ' ' \o E‘. Asparagine ,0 —CH —C (Asn) ' \NH, CH3 H2 H2 l+ Ho—C —-C —ril——CH3 Choline CH3 Phenylalanine (Phe) /CH=CH\ F —CH"C'\CH—CH’ / : o=c s Prollne \(fH'E‘CHKCH (Pro) N_=Z_CH‘/ ‘ ' / a CH / \ Tryplophan "CHI—fi—‘i f“ (Tm) cn/c\ ICH w \NH CH/ Sarina —cu,-OR (Set) Threonine _CH<CH’. (Thr) OH —r‘ Exam 3-06 Biology 131, Examination 3, Spring, 2006 Name (PRINTED IN BLOCK LETTERS) Signature Enter your name in the spaces above and also on the following pages. Answer the questions in the space provided; do not write on the back. Write or print legibly. If you wish to retain the possibility of a re-grade, you must write in ink. Check p.2 for chemical structures. D. Holoblastic Holoblastic Discoldal Superficial cleavage cleavage cleavage cleavage r fi’k t6; Blastocoel l. (10) In the diagram above, what organism (general or specific name OK) is represented by each of the letters A—D? A. SCk'uY‘cLuivx Sliecidmoelerm) B lime (amphibian) C chm glam}! D PM; a; (magenta. we”) What are the cells marked E, and what will they become in the adult? 0 Cc“ ~muu‘Jwt M96914. _ TL 9",“ aw%(:3§s (aymiiwzuflu. 3 Exam 3-06 Name (PRINTED IN BLOCK LETTERS) 2. (15) (A) 100 a. Shown at left is a recording of the membrane potential of a sea urchin egg during fertilization. What is the function of the depolarization? 0 20 40 60 80 Seconds m flak, MOP/WM W W L4.H""‘ 750” b. The sea urchin embryos shown at lefi have been stained for DNA, which makes white spots in the cells. The embryos in panel B were derived from eggs that were fertilized in normal sea water. The cells in panel C were derived from eggs that were fertilized in Na+— free sea water. What are the white spots in the cells marked 1 and 2 (as well as other cells in C), and what is the explanation for them? ‘ : m wow FAA—14 woe“ . MMW’mMVQWJ ' mine/cw Na-‘c- ngn4meflxglii} M +‘\~2 [VJ-l. 0. During sea urchin egg fertilization, what changes occur in Ca2+ in the egg’s cytoplasm and what is the consequence of that change in Ca2+? Be sure to connect your answer here to the issue A “make Wot Ca1I‘WOQ1— NM m Dim/v50“? 97“ 75500.; 0‘ M'liéh‘m. CmA—rwfl W W‘ Exam 3-06 Name (PRINTED IN BLOCK LETTERS) 3. (5) On the diagram to the right, 5 points of the control of gene expression are indicated. Which one is the point of control for the expression of the hunchback gene by bicoid in the Drosophila embryo? Number 5' Which one is the point of control for the expression of the bicoid gene in the Drosophila egg? Number 1 You must get both correct to get any points. Blastocoel _ _ _ Presumptive 4. (5) The dragram r1ght deplcts epidermls , / the transplantation of the dorsal ‘ ' “ lip of the blastopore of one amphibian embryo to the ventral side of another embryo. Describe briefly the subsequent development of the host embryo. W Presumptive notochord \ ~ Presumptive ‘ ; ( somites Primary Dorsal Presumptive in vaginmion blastopore endoderm EXam 3-06 Name (PRINTED IN BLOCK LETTERS) 5. (5). Identify the structure 3 (from an adult) in A above. What are the cells that make up A3 and what is their function? What is the embryological origin of the cells in A3 and what number in B identifies the origin. Structure A3 Dmg flflfl Identity of the cells and their function? Raw mi“: who“ “WM Embryological origin and its number in B? W m -C«~m~. m MM W (alszmhwbamfi). Exam 3-06 Name (PRINTED IN BLOCK LETTERS) A) 4--mm embryo (B) 4.5-mm embryo ' (C) 5-mm embryo (D) 37—min embryo b 6. (5) Shown above are four stages of chick embryo development at the same location in th embryo. Discuss the process that is occurring. Be sure to mention what the region marked “a” is developing into, what earlier embryonic structure “a” is derived from, what the structures marked “b” and “c” (frame D) are or will become, and what is the source and recipient of the If, m» » ‘I \ M‘“ MM W, a M MWMM \MMhahWWLrMM «:2.th “2 wawaw . yma 7. (5) Shown above are a wild-type Drosophila larva and a mutant Drosophz'la larva. Indicate which is which, and name the mutant. If you had eggs from both wild-type and mutant available, what manipulation could you do that would cause the mutant to develop into a wild type embryo. You may use a diagram, but be sure to use enough words so that your meaning is clear. m QM Is Bicm‘o MCI} Mi“ nwfiw). NW w mdm, WM Ma’rer‘QW“ (gnd‘MH/‘Ka‘ 57cm“? WRNH) §rM m Wm '01 V)“ WTQ—Ar‘ 26.3, Magufifim m mmwmva a Maya—a3}. 7 Name (PRINTED IN BLOCK LETTERS) 8. (15) Exam 3-06 +50 Depolarizing Repolarizing 0 phase phase Resting suite Undershoot @ Resting state 400 1: Shown above is an action potential, and its phases are lettered A-D. On the left are shown four states of the two ion channels that underlie action potentials, and they are numbered 1- 4. The two channels are indicated by a and [3. Match the action potential phase with the channel state (4 points). A. A B. ff C. l D. 3 What ion moves through each channel and in what direction (from in to out or from out to in) (4 points) (1. (24+! M 4“ 1 ‘1 Bk? In 40 GJ What is the approximate time of the segment on the action potential graph indicated by “t” (2 points) I mg Exam 3-06 Name (PRINTED IN BLOCK LETTERS) 9. (5 points) Suppose that the concentration of Na+ in an axon is 10 mM. If the concentration of extracellular NaJr were lowered from the usual 100 mM to 10 mM, what would be the maximum (most positive) possible value of an action potential? Show your reasoning and support it with appropriate calculations. 44—2. Nu equilibriwm «pile/dad) VIM. VN 2 S?-«V leggy”: ° 1‘ Syfiv ’03 Ime a. 10. (5) The many inputs into a neuron’s dendrites are summed in the spike initiation region (the part of axon where it joins the cell body). What one key thing must occur in the spike initiating region of a neuron in order to produce an action potential? +W a m M MAMA MAM wows» t» 11. (5) Explain how an action potential leads to the release of acetylcholine at the synapse of the neuromuscular junction. A diagram will be useful, but use enough words so that your understanding of the process is clear to the grader. Do not over-answer the question. JLL ' a.” ca ‘9 000600 C 23- 1k Mam 499‘le MW WV Exam 3-06 Name (PRINTED IN BLOCK LETTERS) 12. (5) B 50‘ .. g A E E 3 L5 0 ------------- ~—o(,«Jcc ———————————————— -- g 0) E 40 E 0) «E 2 ~1>‘c—‘Amwet \j ___.——§—->.. "4 g g Menuhé‘é “so: ‘0 g Above are three curves that show an action potential, and the underlying conductances of Na+ and K“. If the action potential is occurring in an area 10 pm2 of membrane, how many open K+ channels are there at the time of maximum K+ conductance? Show on the graph how you get the necessary starting number for your calculation. What is the membrane potential at the time of maximum K+ conductance? 1‘- : lo/Mmm' = 25-0 C—LGJAMJQ. Do not write below this line. 10 ‘s i ,a Exam 3-06 Name (PRINTED IN BLOCK LETTERS) 13. (15) In answering the following questions, make reference to the numbers on the diagram as needed. Do not answer more than is asked for in the question. If you think you need more than the space provided, you are probably over-answering the question. The question is continued on p. 12. a. (2) What cell is structure 1 part of and where is the cell body? \ - thMfiaqufl/Hfi/MM 9 £5“ .52 M - b. (2) What is contained in the vesicles in structure 1? (Name and chemical structure) Manama (ML) \ 4 o)“O\ /N\ (Ste Pip) c. (3) What are the consequences of the release of the contents of the vesicles into the space 2? Restrict your answer to the events that occur up to point 3. Be sure to identify the cell in which these events are occurring. A Ck Mmh'io M w ()Qpéamo WW. TWA W WW’ XVH (Nags/ital 717% W. DWon-AiéwA—NM m CMJ—tm W10 kw M W ll Exam 3-06 Name (PRINTED IN BLOCK LETTERS) 13 (continued) (1. (2) Which structure is the sarcoplasmic reticulum and What is its response to the signal that originates at 2? 5R I» 3.1?qu Ll), owa X15 WM ’5‘» “l’LLa/oéw‘mzjaq-AMQQ A1 Art Avam 01% Ca slim/~th- M W Ca 6. (3) What number in the diagram indicates troponin and what is troponin’s role with regard to tropomyosin, myosin and actin (be sure to indicate the numbers of these molecules)? . (S) le-Avwlo. (gas M gum?“ Zita Imam». ? rWWMC1). TM expo/m. 2514’ mamuwlm‘mku; (n1 WWQ, )WQ/Qflmi“} 7&4 VHWK K3) Ibo Wulooxi‘» #4 mod-in. f. (3) What process does 9 indicate and what is the importance of the process? ‘ Sgrw“ W M a. Mac/AMA: 43m. “iva-JWWQ 2&4 ~ cl £nc9acaA-(4. flan Cal 4-way» W W M Mia ‘K’L-z $9 «rim m Cara—Afloat. 12 ...
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This test prep was uploaded on 04/18/2008 for the course BIOL 131 taught by Professor Robinson during the Spring '08 term at Purdue.

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Bio test 3 practice 5 - Exam 3-06 Biology 131 Examination 3...

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