BMB_460 Final Paper - BMB 460 p53 "The Guardian of the...

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BMB 460 p53 “The Guardian of the Genome” Abstract I chose to write my paper over gene expression with p53 in breast cancer. I found this article to be very interesting and also decided to write about it because of the tremendous affect breast cancer has on our society. I figure that with a better understanding of breast cancer and the gene expression involved with it I can take this knowledge and use it in the fight against breast cancer. The article was a refinement of the p53-associated gene list. By updating this list and showing that the loss of p53 had an impact on not just the genes it’s directly associated with (such as GATA3 and CDC2), but possibly with genes that are down from the regulation site for p53 associated genes. This is important as these genes are known to be oncogenes and therefore the study of them and the influence of p53 on their up and down regulation is critical to the understanding and possible treatment of breast cancer. They accomplished this analysis by testing the gene expression of p53 dependant genes on four different cell lines (ZR-75- 1, MCF-75, and 2 other immortalized epithelial cell lines) by transducing them with p53- RNAi (hairpin structures) that were grown up in microorganisms which were lysed, and the supernatant collected and purified. Cell lines that showed the expression of the p53- RNAi were selected for further analysis. The cells were then separated into 2 groups, those that were treated with DOX and those that weren’t. These groups were then analyzed to see if the loss of p53 had an affect on gene expression and which genes were affected. This data was then compared to data that was collected from breast cancer patients to find correlation of gene expression between the groups. This created a flaw
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however, because the expression in the experiments only showed four different cell lines whereas the data collected from patients was from a much more diverse set of “cell lines”. From all this data a list was put together about the genes affected by p53 loss in both in vitro and in vivo data sets. This list and the data collected were also analyzed by some different statistical methods. From these experiments they showed that the loss of p53 had an impact on all four cells lines as well as breast tumors. Each cell line showed different responses to the loss of p53 but the cell types showed similarities on how they were affected. I found that this article was very interesting and pertinent to the battle with breast cancer by finding what genes are involved in breast cancer and how the loss of p53 can affect the expression of these genes. Background p53 was first discovered in 1979 by a group of researchers found an unknown protein in a set of serological experiments of animals with varying types of cancer. They found that the hosts had antibodies to the protein in their serum and they initially concluded that the protein was an oncogene. Years later more researchers found that this protein was present in most forms of cancer, but it was only an oncogene in its mutant
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This note was uploaded on 04/18/2008 for the course BMB 460 taught by Professor Gunderson during the Spring '08 term at University of Maine Orono .

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BMB_460 Final Paper - BMB 460 p53 "The Guardian of the...

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