BioChem2 - BMB 323 Isolation and Characterization of Bovine...

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Unformatted text preview: BMB 323 Isolation and Characterization of Bovine Milk -Lactalbumin Abstract The isolation and purification of the -Lactalbumin protein was achieved in this lab by a combination of centrifuging, pH adjustments and by affinity chromatography. The final concentration on the -Lactalbumin wasnt attainable however, due to errors in the experimental procedure. Results Buffer A Buffer B Tube Absorbance 11-0.095 12-0.079 13-0.94 14-0.076 15-0.052 16 0.53 17 0.195 18 0.02 19-0.056 20-0.092 Bradford Protein Assay Tube 1 2 3 4 5 Y-globulin (l) 10 20 40 60 80 H20 (l) 90 80 60 40 20 Bradford (l) 1000 1000 1000 1000 1000 Total 1100 1100 1100 1100 1100 Absorbance .009 .038 .054 .065 .075 Tube Absorbance 1 0.189 2 0.193 3 0.202 4 0.194 5 0.191 6 0.193 7 0.223 8 0.219 9 0.205 10 0.202 Bradford Protein Assay 0.01 0.02 0.03 0.04 0.05 0.06 0.07 0.08 0.001 0.002 0.004 0.006 0.008 mg of protein Absorbance at 595nm Absoption of -Lactalbumin-1.2-1-0.8-0.6-0.4-0.2 0.2 0.4 0.6 0.8 1 2 3 4 5 6 7 8...
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BioChem2 - BMB 323 Isolation and Characterization of Bovine...

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