BioChem4 - BMB 323 Isolation and characterization of Plant...

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BMB 323 Isolation and characterization of Plant Pigments Abstract The color of plant leaves comes from a combination of chlorophylls and carotenoids inside the plant cells. These pigments were isolated out of spinach leaves and then characterized by paper chromatography. The amount of pigments isolated out form the spinach was also quantified by absorption techniques. Results wavelength Blank Sample Final 662 0 0.157 0.157 645 0.46 0.552 0.092 450 0.765 1.032 0.267 Distance (cm) Rf Solvent 5.5 1 Light green 2 0.363636 Dark green 1.4 0.254545 Yellow 3 0.545455 Dark Green Absorbance Wavelength 0.6 450 0.22 660 Light Green Absorbance Wavelength 1.05 450 0.8 660 Yellow Absorbance Wavelength 0.3 450
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C a = 11.24(.157)-2.04(.092)= 1.577 μg/ml C b = 20.13(.092)-4.19(.157) = 1.19413 μg/ml C a+b = 7.05(.157)+18.09(.092) = 2.77113 μg/ml C x+c = 1000(.267)-1.90(1.577)-63.14(1.19413) = .88133 μg/ml 214 Discussion After the grinding of the spinach leaves with the sand and acetone (both used to help extract the pigments from the leaves) a dark green solution was collected. After this
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BioChem4 - BMB 323 Isolation and characterization of Plant...

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