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BioChem5 - BMB 323 Isolation and characterization of Plant...

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BMB 323 Isolation and characterization of Plant Pigments Abstract Serum albumin is an essential part of the transportation of nutrients to peripheral tissues. By having a special binding affinity to certain molecules it can bind to essential nutrients and flow to anywhere in the body via the blood stream. The dynamics of this binding process can be isolated and observed via a technique called gel filtration. By binding these proteins with phenol red dye separation can be achieved by gel filtration and the rate of such can be calculated. Results Tube # Group # Peak 1 (A 520nm ) Peak 2 (A 520nm ) V [L] V/[L] 1 1 0.281 9.854 0.12 1.264 3.1144 2 7 0.563 20.421 0.232 5 2.53 2.59915 3 8 0.657 23.78 0.464 4 5.06 1.537048 4 4 1.4 (hypotheti cal) 56.38 0.627 8 7.611 0.788676 5 5 1.69 79.133 0.722 4 10.18 2 0.355408 Absorptions 0.027 0.064 0.242 0.647 0.038 0.07 0.252 0.582 0.042 0.077 0.261 0.537 0.054 0.08 0.299 0.458 0.039 0.083 0.361 0.349 0.04 0.093 0.405 0.254 0.037 0.101 0.479 0.207 0.038 0.113 0.505 0.158 0.04 0.161 0.571 0.125 0.043 0.21 0.604 0.118 0.053 0.224 0.637 0.099 0.053 0.233 0.659 0.08 0.057 0.243 0.668 0.075
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Absorptions 0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 1 4 7 10 13 16 19 22 25 28 31 34 37 40 43 46 49 52 Sample Absorption red dye bound =__ 0.563_____ = 0.563 *100 = 2.683% 0.563 + 20.421 20.984 0.10/1.0 * 26mM = 2.6mM.
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