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Chem 322L Lab 1 report.docx - Experiment No 1 – Tools of...

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Experiment No 1 – Tools of the Trade: Dilution, Calibration, and Error AnalysisName:University of South CarolinaChem 322LTA:February 7, 2022
AbstractThe purpose of this laboratory experiment was to solve for an unknown concentration ofsucrose solution by substituting the density of the unknown sucrose solution into the equationscalculated for both the parallel and serial solutions.After finding the unknown concentration, the slopes and y-intercepts of both dilutiontechniques were compared to the class average, and a t-test was used to evaluate if the dilutiontechniques differed from the class average for each dilution at the 95% level of confidence.IntroductionThis experiment required a knowledge of modern tools, used predominantly forcalculating mass and volume of solutions, preparing calibration curves based on data calculated,and using data collected to calculate density of an unknown solution to compare previouslycollected data, in this case, the rest of the class averages.The relationship between density and temperature was observed and noted that densitywas indirectly proportional to the temperature. A total of five sample were obtained for theparallel dilution as well as five samples for the serial dilution. In a parallel dilution, aconcentrated stock solution is started with and new solutions are made with several differentmilliliters of stock solution to different flasks, all ending with the same volume. In a serialdilution, a concentrated stock solution is diluted in solution, but the diluted solutions that comeafter that are taken from the previous diluted solution, decreasing in solution concentration foreach new sample.ProcedureFour data tables were made to measure & record the calibrations of a 10-100 μL pipette, a100- 1000 μL pipette, and the percent concentration of an unknown sucrose solution in a paralleldilution and a serial dilution. The two pipettes were calibrated by first taring the balance to zero.A weigh boat was placed on the scale, then tared to account for the mass of the water. Utilizingboth Eppendorf pipettes capable of measuring 10-100 μL and 100-1000 μL respectively, the massof any further added water and the set volume were recorded and steps repeated a total of threetimes per volume measured. After the data calculation for the 10-100 μL pipette at volumes: 10,25, 50, 75, and 100 μL, and the 100-1000 μL pipette at volumes: 100, 250, 500, 750, and 1000

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Term
Spring
Professor
NoProfessor
Tags
Chemistry Department

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