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10.30 - Sabban - Transcriptional Control and Gene Expression

10.30 - Sabban - Transcriptional Control and Gene...

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Transcription Control and Gene Expression Dr. Sabban 10/30/2007 1 2 Control of Transcription in Bacteria Lac Operon 3
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4 5 6
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7 8 The Lac operator showing the nearly perfect inverted repeat 9
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10 11 CAP – Catabolite activator protein (also called CRP – cAMP response protein) Binds inverted repeat at -61 of lac operon, facilitates binding of RNA polymerase Increases transcription about 50-fold Positive regulation 12
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13 14 15 Groups in DNA available for binding (shown in red)
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Helix-turn helix motif (yellow) 16 Trp Operon Trp repressor (also regulation by attenuation) Purine Operon Pur repressors, only binds DNA with corepressor (Guanine or hypoxantine), thus active under normal (not enriched) conditions 17 Other Examples 18
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19 20 Need to consider chromatin structure Chromatin remodelling Examples of CREB and estrogen receptors 21 Control of Transcription in Eukaryotes
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Sites hypersensitive to the non-specific DNase I are found within 1 kb of the start site of active genes
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