6. Wu - Review - 1 Review •Nucleotide...

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Unformatted text preview: 1 Review •Nucleotide Synthesis/Degradation 1. Name two di ff erences in de novo purine synthesis between prokaryotes and eukaryotes. ! P: individual genes; E: fused proteins ! P: de novo folic acid synthesis; E: from diet 2. Name one drug developed based on the above di ff erence. ! trimethylprim (works in proks not euks) 3. Name two di ff erences in de novo pyrimidine synthesis between prokaryotes and eukaryotes a. P: individual vs. E: fused b. rate-limiting, controlling steps are di ff erent P: aspartate transcarbamoylase E: CPSII 4. Name two similarities & two di ff erences in de novo purine and pyrimidine synthesis ! Similarities: ! both highly energy-dependent ! overlap in AA requirements (Glutamine & Asparagine) ! Di ff erences: ! Pur: make nucleotides (IMP) using PRPP ! Pyr: make base (orotic acid) ! Pur: can make ATP, GTP ! Pyr: can’t make TMP Lecture 1 - Slide 9 1. What is the e ff ect on de novo purine synthesis for an individual su ff ering from a deficiency in purine or pyrimidine salvage enzymes? •no salvage enzymes = excess PRPP which feedforward activates amidotransferase therefore increased purine biosynthesis 2. Name a disease characterized by deficiency in a salvage enzyme. •Lesch-Nyhan Syndrome 3. Propose a mechanism that underlies the clinical manifestation. •stunted growth, retarded, self- mutilation b/c cannot do de novo purine synthesis Lecture 1 - Slide 14 1. Mycophenolic acid is an inhibitor of the enzyme IMP dehydrogenase. What would the addition of mycophenolic acid to tissue cultured cells be expected to do? acid to tissue cultured cells be expected to do?...
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This note was uploaded on 04/18/2008 for the course PHYS 1010 taught by Professor Thompson during the Fall '07 term at New York Medical College.

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6. Wu - Review - 1 Review •Nucleotide...

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