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6. Wu - Review - Review Nucleotide Synthesis/Degradation 1...

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1 Review •Nucleotide Synthesis/Degradation
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1. Name two di ff erences in de novo purine synthesis between prokaryotes and eukaryotes. ! P: individual genes; E: fused proteins ! P: de novo folic acid synthesis; E: from diet 2. Name one drug developed based on the above di ff erence. ! trimethylprim (works in proks not euks) 3. Name two di ff erences in de novo pyrimidine synthesis between prokaryotes and eukaryotes a. P: individual vs. E: fused b. rate-limiting, controlling steps are di ff erent P: aspartate transcarbamoylase E: CPSII 4. Name two similarities & two di ff erences in de novo purine and pyrimidine synthesis ! Similarities: ! both highly energy-dependent ! overlap in AA requirements (Glutamine & Asparagine) ! Di ff erences: ! Pur: make nucleotides (IMP) using PRPP ! Pyr: make base (orotic acid) ! Pur: can make ATP, GTP ! Pyr: can’t make TMP Lecture 1 - Slide 9
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1. What is the e ff ect on de novo purine synthesis for an individual su ff ering from a deficiency in purine or pyrimidine salvage enzymes? •no salvage enzymes = excess PRPP which feedforward activates amidotransferase therefore increased purine biosynthesis 2. Name a disease characterized by deficiency in a salvage enzyme. •Lesch-Nyhan Syndrome 3. Propose a mechanism that underlies the clinical manifestation. •stunted growth, retarded, self- mutilation b/c cannot do de novo purine synthesis Lecture 1 - Slide 14
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1. Mycophenolic acid is an inhibitor of the enzyme IMP dehydrogenase. What would the addition of mycophenolic acid to tissue cultured cells be expected to do?
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