BCH 463 Exam_final 2010 - NAME KEY Final Examination for...

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NAME KEY Final Examination for BCH 463 Wednesday, May 12, 2010 This examination is scheduled for 1 hour and 50 minutes, from 12:10-2:00 pm. All problems in this section are worth 3 points each. Some physical constants: h = 6.62 x 10-34 Js me= 9.1 x 10-31kg mp= 1. 67 x 10-27kg c = 2.99 x 108m/s ao= 5.29 x 10-11m γp = 26.75 x 107T-1s-1R = 8.31 J K-1mol-1F = 9.6 x 104J V-1mol-1RT = 2.47 kJ mol-1at 298 K 2.3 RT/F = 58.6 mV at 298K 1 eV = 1.60 x 10-19J =1.0541034Jse = 1.602 x 10-19C ε0= 8.854 x 10-12J-1 C2 m-1RH= 109 677 cm-1μN= 5.05 x 10-27JT-1μB= 9.27 x 10-24JT-1
1. What is the extinction coefficient for a sample with an absorption of 0.2 in a 1 cm cuvette and a concentration of 1 mM?
2. How many photons are found in stimulated emission compared to spontaneous emission
3. If you measure the fluorescence peak at 900 nm where would you expect to find the absorption peak?

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