Lab 5 (2) - Lab 5 Calibration Methods and Introduction to...

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Lab 5: Calibration Methods and Introduction to UV-Visible SpectroscopyPre-Lab Questions1.(1mg Solute1LSolvent)(100.0ppm1)= 100 mg solute, which is 0.1000g(2mL HCl100mL HClsolution)(1000mL HCl solution1L)= 20 mL concentrated HCla.Weigh approximately 0.1000g of the pure ASA and place it in a 1000mL volumetric flask.b.Measure 20mL of the concentrated HCl and add it intot he volumetric flask.c.Fill to the line with DI water2.Concentration (ppm)Volume Added of ASA (mL)00.0040.4060.6080.80101.00151.50202.00100Fill to line3.1 ppm = 1 mg/LC1V1 = C2V2325 ppm × x = 10ppm × 10 mL= (10ppm×10mL325ppm)= 0.3076 mL= 308 µL
Post Lab Questions1.Table of results after Grubb’s testThe values in red are the excluded values that failed the Grubb’s Test. The unknown and unknown + spike were not tested since it was assumed that these would be different for each individual.2.ConcentrationAverageStandard Deviation00.000012500.000540.14710.00623240460.22340.01059012380.29120.006749378100.36070.008622727150.54280.008872778200.72130.0166167251002.48160.164260062
05101520250.00.10.20.30.40.50.60.70.8f(x) = 0.04x + 0R² = 1Concentration vs. AbsorbanceConcentration (ppm)Average Absorbance
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