protein kinase A

protein kinase A - 6/7/08 8:39 PM PKA adds a phosphate to...

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6/7/08 8:39 PM PKA adds a phosphate to things. PKA takes ATP and converts to ADP. Catalytic subunit: have a protein kinase domain, the part with catalytic activity. o 2 subdomains N-terminal (N lobe) mostly beta sheet C-terminal (C lobe) mostly alpha helix. the ATP sits btw these 2 subdomains. Schematized PKA structure o N lobe on the top, C lobe on the bottom. o Activation loop (orange) bound to ATP Some other protein kinase phosphorylate the activation loop. B/c a phosphate stuck on the activation loop, changes the conformation of activation loop, allow the lobes to come together, ATP position correctly, substrate bind on top of the activation loop. the activation loop provides a platform upon which the substrate sits has a different conformation when the kinase is active.
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For diff kinases, the activation lops themselves are not very conserved. o Once phosphorylated, alter conformation of activation loop. o alphaC involves in positioning ATP. alphaC: contain E91 (glutamate; neg charge residue); red interacts with K72 (lysine; blue) see the schematized PKA figure o the yellow loop is the substrate. o
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protein kinase A - 6/7/08 8:39 PM PKA adds a phosphate to...

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