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xs96633 - 3 w r"_Biological Sciences 103\Iame...

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Unformatted text preview: 3/ w r" __Biological Sciences 103 \Iame _ SECti‘On 1 Last. First MI Instructor: Roy H. Doi “MW 3609 IslMidtemi ' N )Verr ber 11,2002 There are 10 pages to this exami lath n including this front page. Please count them to make sure they are all present. Each question has only one best 'con tplete answer. Each question is worth 5 points Be sure to write your name and ]D n' imber on the Scantron sheet . Be sure to use a #2 lead pencil tc fill in your answer. Be sure to turn in this exam along with your Scantron Sheet. Honor Code: My signature below affirms that I wrote this exam in the spirit of the honor system of UC Davis. I neither received nor furnished any "help" during the exam, nor did I use any unauthorized references. Signature I, ____________________, ID#_____________ authorize the University to publicly distribute this graded exam (e. g. handed out in class or left in a bin for me to pick up). All questions will be worth 5 points each. There is we best/complete answer for each question. 1. During fatty acid oxidaton a. The enzymes for fatty acid oxidation in animal cells are located in the mitochondrial matrix. b. Fatty acids must undergo three enzymatic reactions to get from the cytosol to the mitochondrial matrix. @ The products of acyl—CoA synthetases are fatty acyl-CoAs, ADP and Pi. a. and b e. a and b and c 2. During each cycle of B-oxidation the following energy rich products are formed: a. one FADH2 and one NADPH + H b. one FADH2 and two NADPH + H 3. two FADH2 and one NADH + H+ one FADH2 and one NADH + H e. none of the above 3. The B—oxidation of linoleoyl—CoA (18:2:cis-A9, cis-A”) to 9 acetyl-CoAs results in the formation of how many ATPs? Assume that any reduced co—factors are oxidized by the electron transport system and that oxidative phosphorylation occurs. Assume that energy is required for the formation of linoleoyl-CoA and this has to be accounted for. Assume that the acetyl- CoAs are not being oxidized further in this question. a. 45 ATPs . 42 ATPs c 5 ATPs . 33 ATPs e .30 ATPs 4, For each round of B-oxidation during fatty acid degradation , there are two steps in which oxidation occurs, the two enzymes involved in these steps are a. acyl—CoA dehydrogenase and pyruvate dehydrogenase b. acyl-CoA dehydrogenase and isocitrate dehydrogenase B-hydroxyacyl-COA dehydrogenase and acyl-CoA dehydrogenase d. B-hydroxyacyl-COA dehydrogenase and acyl-CoA acetyltransferase e. none of the above 5. In order for B-oxidation to occur during oxidation of unsaturated fatty acids, the enoyl-CoA hydratase requires a. a cis configuration of the H between Cl and C2 b. a cis configuration of the H between C2 and C3 @ a trans configuration of the H between C2 and C3 . a trans configuration of the H between C1 and C2 e. a trans configuration of the H between C3 and C4 6. One of the following compounds is important in the positive transport of fatty acids into the mitochondrial matrix. a. malonyl-COA b. palmitoyl-CoA @ camitine . acetyl—CoA e. NADPH 7. The following compounds or factors have a negative effect on the transport of fatty acids into the mitochondria malonyl—CoA and high concentrations of acetyl-CoA b. low [NADH/NADU ratio low energy charge of the cell c. d. oxaloacetate e. citrate 8. The reducing power required during fatty acid synthesis can be derived from oxidation of glycerol-phosphate dehydrogenase the pentose phosphate pathway (PPP) malate dehydrogenase reaction . the reaction carried out by the malic enzyme .bandd 9. The major end products of triacylglycerol degradation are acetyl-CoA (1) (from fatty acid oxidation) and glycerol(2), bib (3) of which can be used in gluconeogenesis in animals. Glycerol is phosphorylated by glycerol kinase14) and can be converted to glucose in addition to being processed through glycolysis Q). The following is false: a. 1 + 2 @ c. 4 d. 5 e. none of the above up?!» 10. During fatty acid synthesis a. the first committed step is the formation of malonyl-CoA. b. malonyl-CoA is synthesized by acetyl—CoA carboxylase from acetyl-CoA and HCO3' biotin is a co—factor for acetyl-CoA carboxylase and can carry C02 ATP is required in the acetyl-CoA carboxylase reaction C. d. .All of the above 11. During fatty acid biosynthesis by the fatty acid synthase complex malonyl-CoA is bound to a cysteine group of acyl carrier protein (ACP). malonyl-CoA is bound to a phosphopantetheine prosthetic group of ACP. caproyl-CoA is bound to ACP after the first condensation step. butyryl-CoA is bound to ACP after the first condensation step. b + d 9999‘!» 12. During the first round of activity by the fatty acid synthase complex a. two moles of NADH are required @ two moles of NADPH are required a condensation step and a hydration step occurs (1. a + c e. b + c 13. For the production of a saturated 16 carbon fatty acid by the fatty acid synthase complex a. 6 cycles of condensation and reduction were required ® the product was palmitoyl-CoA or palmitate plus CoA c. 6 C02 were produced and 6 ATPs and 12 NADPH + H+ were required (1. 6 malonyl-CoAs were required e. a + d 14. The following TCA intermediate played a critical direct role to shuttle acetyl-CoA to the cytosol from the mitochondria a. succinate b. malate Q citrate . pyruvate e. isocitrate 15. Malic enzyme plays a key role in the cytosol during fatty acid synthesis by providing the following compound for fatty acid synthesis. a. malate b. pyruvate c. ATP NADPH + H+ e. Oxaloacetate 16. The regulation of glycolysis and gluconeogenesis or the regulation of B—oxdation of fatty acids and fatty acid synthesis is an example of a. metabolic regulation @ reciprocal regulation of pathways . futile cycling d. translational regulation e transcriptional regulation 17. The end products of the oxidative phase of the Pentose Phosphate Pathway are: a. NADPH, Ribulose-S-Phosphate, O2 and xylulose-S-Phosphate b. NADH, Ribulose-S-Phosphate and 02 c. NADH, Ribulose-S-Phosphate, CO2 and xyluose-S-Phosphate © NADPH, Ribulose-S-Phosphate and CO2 e. NADH, Ribose—S-Phosphate, CO2 and xylulose-S-Phosphate 18. Ketose is the of carbon units, while aldose is the of _ carbon units. a. acceptor, two, donor, three b .donor, three, acceptor, two donor, two, acceptor, two . acceptor, three, donor, two e. acceptor, three, acceptor, two 19.When transketolase transfers _ carbons in the form of activated , the carbon donor becomes a(n) . a. Three, glycoaldehyde, ketose. Two, glycoaldehyde, aldose c. Three, dihydroxyacetone, aldose d. Two, dihydroxyacetone, ketose 6. None of above 20.Which of the following enzymes catalyzes an “irreversible” reaction. . Epimerase @ Glucose-6—Phosphate Dehydrogenase . Transketolase d. Transaldolase e. Phosphopentose Isomerase 21.If Ribulose-S-Phosphate is not required for the synthesis of co—factors, the non-oxidative phase products _ and _ can be used for to generate _. ,Fructose—6—Phosphate, Glyceraldehyde-3-Phosphate, glycolysis, ATP. . Fructose-6—Phosphate, Glyceraldehyde-3-Phosphate, beta—oxidation, NADPH. c. Fructose—6-Phosphate, G1ucose-6-Phosphate, beta-oxidation, ATP. d. Fructose-6—Phosphate, Glucose-6-Phosphate, glycolysis, ATP. 6. Fructose—6-Phosphate, G1ucose-6-Phosphate, beta-oxidation, NADPH. 22 In the Pentose Phosphate Pathway, the epimerase enzyme converts _ to a. Ribulose-S-Phosphate, Ribose-S-Phosphate b. Ribose-S-Phosphate, Fructose—6-Phosphate Xylulose-S-Phosphate, Ribulose-S-Phosphate . Xylulose—S-Phosphate, Ribose-S-Phosphate e. Xylulose—S—Phosphate, Fructose-6-Phosphate 23.Which of the following is not a principal substrate for Gluconeogenesis: a. lactate b. glycerol c. alanine malate e. pyruvate 24. The glycolytic step involving the enzyme has a standard free energy change of and is bypassed in gluconeogenesis by the enzyme a. Phosphofructokinase, -14.2, PEP carboxy kinase. b. Phosphofructokinase, -l6.7, Glucose-6—Phosphatase. ©Hexokinase, -l6.7, Glucose-6-Phosphatase. . Hexokinase, —16.7, Fructose-1,6-bisphosphatase e. Hexokinase, -14.2, Fructose-1,6-bisphosphatase 25. The enzyme __ catalyzes the conversion of * to _, which requires _ and _ a. .Pyruvate carboxylase, 0AA, malate, ATP, C02. b. Pyruvate carboxylase, pyruvate, 0AA, acetyl-CoA, NADH. Pyruvate carboxylase, pyruvate, 0AA, acetyl-CoA, C02. . Malate Dehydrogenase, malate, 0AA, ATP, C02. e. Malate Dehydrogenase, OAA, malate, ATP, NADH. 26. Glucagon reduces the level of , which glycolysis and gluconeogenesis. a. F-2,6-BPase—P, inhibits, favors. b. Fructose—6-Phosphate, inhibits, favors. Fructose—2,6-BP, inhibits, favors . F—2,6-Bpase-P, favors, inhibits e. Fructose-2,6-BP, favors, inhibits 27 .In gluconeogenesis, the enzyme PFK2-OH converts _ to _ which __ glycolysis. a. Fructose-2,6-BP, Fructose—6-P, favors. b. Fructose-2,6-BP, Fructose-1,6-BP, favors. c. Fructose-2,6-BP, Fructose—1,6-BP, inhibits @Fructose-6-P, Fructose-2,6—BP, favors e. Fructose-6-P, Fructose-2,6-BP, inhibits 28. Phosphorylase a degrades glycogen to , which can be converted to glucose by a or to glucose-6—phosphate by a a. Glucose-l-phosphate, phosphoglucomutase, phosphatase. @Glucose- 1 -phosphate, phosphatase, phosphoglucomutase. c. Glucose-l-phosphate, phosphatase, isomerase. d. Glucose— 1 -phosphate, isomerase, phosphatase. e. Glucose—l—phosphate, phosphoglucomutase, epimerase. 29. Which of the following items is not absolutely required for the light reaction of photosynthesis in higher plants? a. chloroplasts . water as the electron donor @ carbon dioxide as the electron acceptor . energy from sunlight e. light absorbing pigments 30. The primary products of the non—cyclic photophosphorylation pathway during photosynthesis in higher green plants are the following: a. ATP and NADPH b. Oxygen 0. Reduced ferredoxin and NADPH d. a. and c. a a. and b. 31. The primary product(s) of the cyclic photophosphorylation pathway during photosynthesis in higher C3 plants is (are) a. oxygen b. NADPH ATP . Reduced ferredoxin e. All of the above 32. How many quanta of light are required to produce 2 moles of glucose based on the following equation and our discussion of quantum requirements for photosynthesis in higher plants: CO2 + 2 NADPH + 2H+ + 3 ATP + 2H20 => C(HZO) + 2 NADP+ + 3 ADP + 3 Pi + 02 + H20 a. 9 b. 36 c. 54 33. If 4 moles of NADPH + H” are formed during photosynthesis in higher plants according to the the following equation and our discussion of the Hill-Bendall Z scheme, CO2 + 2 NADPH + 2H+ + 3 ATP + 2HZO => C(HZO) + 2 NADP+ + 3 ADP + 3 Pi + 02 + H20 a. PSI is excited by 18 quanta of light. b. P811 is excited by 10 quanta of light @ PSI is excited by 8 quanta of light . PSI is excited by 10 quanta of light e. P811 is excited by 20 quanta of light 34. During cyclic photophosphorylation in higher green plants a. Two quanta of light are required for each ATP that is synthesized, because of the difference in redox potential of ferredoxin and cytbf in PSI. b. Three quanta are required during passage of electrons from ferredoxin to cytbf for each ATP that is synthesized and that is required to fill the electron hole in PSI . 0. Two quanta of light are required in P811 to fill the electron hole that is made in PS I during the synthesis of two ATP. d. The passage of electrons from PS I to PS 11 is essential for the synthesis of ATP and for filling the electron hole in PS 1. One quantum of light is required for PS I during cyclic photophosphorylation , because the difference in redox potential between cytbf and ferredoxin provides enough free energy for the synthesis of one ATP. 35. The following statements concern photorespiration in C3 plants; select the one best/complete answer a. the enzyme Rubisco carries out this reaction. b. the products of photorespiration are phosphoglycolate and 3-phosphoglyceric acid c. both 02 and CO2 fixation to ribose-l,5-bisphosphate can occur concurrently d. photorespiration constitutes a significant waste of energy for the plant all of the above. 36. The carboxylation phase when one C02 is fixed during photosynthesis in C3 higher green plants results initially in the formation of a. two moles of 2-phosphoglyceric acid b. one mole of D—ribulose-1,5-bisphosphate c. oxaloacetate and acetyl-CoA two moles of 3—phosphoglyceric acid e. none of the above 37. If 12 C023 are fixed in the carboxylation phase of photosynthesis, the C3 plant would have to use 24 NADPH + 24 H+ + 36 ATP c. 36 ATP +18 NADPH +18 H+ d. 24 ATP + 24 NADPH + 24 11* e. 24 NADPH + 24 H” + 18 ATP ‘12 NADPH + 12 H+ + 18 ATP 38. The enzyme that carries out CO2 fixation in C3 plants during the carboxylation phase is called a. ribulose-S-phosphate kinase b pyruvate carboxylase ribulose- 1,5—bisphosphate carboxylase . malic enzyme e. phosphopentose epimerase 39. During the C3 plant Calvin pathway, the function of the NADPH + H+ made during the light reaction is a. reduction of oxaloacetic acid to malate b. reduction of 1,3-bisphosphoglyceric acid to dihydroxyacetone phosphate c. reduction of pentose-S-phosphates to their alcohol levels eduction of 1,3-bisphosphoglyceric acid to glyceraldehyde-3-P e. none of the above 40. Which of the following transaldolase reactions will yield sedoheptulose—7-P and glyceraldehyde-3—P a. fructose—6-P + glyceraldehyde—3-P @ - rythrose—4—P + fructose—6-P . ribulose-S—P + xylulose-S—P d. erythrose—4-P + ribose—S-P e. ribulose-S—P + ribose-S-P END OF EXAMINATION 10 ...
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