MG-section_week_7-answers - 7. TA:MikeGavio #16,17,18 1...

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7.03 Recitation -- Week Seven TA: Mike Gaviño Email: [email protected] Main concepts from Lectures #16, 17, 18: 1. Transformation is a process by which foreign DNA is directly introduced into babcterial cells, rather than using a phage (as in transduction) or the F plasmid (as in conjugation) as a vector. a. Is the DNA used for bacterial transformation circular or linear? What sequences are necessary for this foreign DNA to propagate and for transformant cells to later be isolated? 2. Recombinant DNA can be generated by using restriction endonucleases along with DNA ligase to “cut and paste” segments of DNA. In conjunction with transformation, recombinant plasmids can be used to introduce specific DNA sequences into recipient bacteria. a. What are genomic and cDNA libraries? Why are these useful? b. What is cloning by complementation? 3. The lac operon is a series of bacterial genes activated only in the presence of lactose or the lactose analog IPTG. Recessive/dominant and cis/trans tests on uninducible and constitutive mutants were used to determine that the expression of genes in the lac operon is negatively regulated when the LacI repressor protein binds to the DNA sequence
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This note was uploaded on 02/06/2009 for the course 7 7.03 taught by Professor Fink during the Spring '09 term at MIT.

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MG-section_week_7-answers - 7. TA:MikeGavio #16,17,18 1...

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