MG-section_week_11

MG-section_week_11 - 7.03 Recitation Section Eleven Main...

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7.03 Recitation -- Section Eleven Main concepts from Lectures #24 -27: 1) We can characterize gene expression and protein-DNA interactions with sequencing technology. To characterize gene expression we turn expressed RNA into DNA, and sequence the resulting DNA. To characterize where a protein binds to DNA, we purify protein-DNA complexes using an antibody, and then release and sequence the DNA to discover where the protein binds in the genome. 2) We can test how likely it is that data from two genes occurred assuming the genes are independent by using a chi-square test with one degree of freedom. If the genes are independent we expect: G2 off G2 on G1 on P(G1)(1-P(G2))total P(G1)P(G2)total G1 off (1-P(G1))(1-P(G2))total (1-P(G1))P(G2)total 3) If we do many comparisons to look for dependent pairs of genes, we need to adjust our uncorrected p-value to get a corrected p-value as follows: corrected p-value = uncorrected p-value x number of comparisons 4) One can characterize single base nucleotide polymorphisms ( SNPs ) genome wide.
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