MG-section-week6-answers

MG-section-week6-answers - 7.03RecitationWeekSix...

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7.03 Recitation -- Week Six TA: Mike Gaviño Email: mgavino@mit.edu OH: M 1-2p, T 2-3p Main concepts from Lectures #13, 14, 15: 1) There are three ways of getting DNA into bacterial cells -- transformation, transduction, and conjugation. Transformation involves plasmids being taken up directly from the environment. Transduction involves the use of phage as a vehicle for the DNA. Conjugation involves DNA transfer through mating. 2) Transposons are short stretches of DNA that hop around. Transposons can be used to create a collection (or "library") of ~5,000 bacteria in which each bacterial cell has a transposon inserted into (and thus disrupting) a different bacterial gene. 3) A phage is a haploid virus that infects bacteria. After infecting bacteria, P1 phage occasionally pack E. coli chromosomal DNA into a new phage head. Such packing mistakes can be used to determine the frequency at which two genes are “cotransduced,” or moved together into a new bacterium by the progeny phage. 4) The F plasmid is an extrachromosomal circle of DNA that can be transferred to F E. coli recipients by mating. An Hfr strain has an F plasmid integrated into its genome; this plasmid can recombine out to create an F' that contains some host genes. Questions based on Lectures #13, 14, 15: Ways to transform bacteria for genetic analysis: Transduction Conjugation How is Donor DNA transferred into a recipient bacteria in this process? Phage lyse a donor strain and with
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MG-section-week6-answers - 7.03RecitationWeekSix...

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