7.05_Problem_Set_1_answers

7.05_Problem_Set_1_answers - 7.05 Problem Set 1 Answers 1.)...

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7.05 Problem Set 1 Answers 1.) Know your amino acids! From Exam 1, 2007 A.) Complete the drawing of the chemical structure of the peptide with sequence: Ser-Thr-Pro-Ala-Thr-Arg-Ile-Cys-Lys as it would be found at pH 6.0. Be sure to indicate the proper ionization states of any relevant chemical groups. (You can draw the structure on two lines if it is too long for a single line.) (8 points) B.) Draw boxes around the amide bonds. Draw an arrow to indicate which one of the amide bonds is most likely to be in the cis-conformation. ONLY SELECT ONE! (2 pts.) C.) Put asterisks (*) next to the side chains that carry formal charges at pH 6. (2 pts.) D.) Put filled circles ( ) next to the side chains that are hydrophobic. (2 pts.) E.) Put crosses ( ) next to the side chains that are not charged but are polar (2 pts.) F.) Draw a dashed circle around the amino acid side chain that is able to form disulfide bonds. (2 pts.) G.) Write the sequence in single letter amino acid code below. (2 pts.)
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2. Understanding Protein Purification a.) Characterize the four types of chromatography and fill in the chart below. I filled in the column for gel filtration as an example. Chromatography Gel Filtration Ion Exchange Ion Exchange Affinity Anion Cation Separates based on: Protein Size Charge Charge Unique Binding Properties Matrix: Porous Beads Polyacrylamide, Dextran Beads with cations attached Beads with anions attached Beads with the protein’s ligand attached Travels slow/ Is bound: Small Proteins Anions Cations Protein of interest Travel fast/ Flows through: Large Proteins Cations Anions Everything else Additional Information: (for example, how to elute bound proteins) Small proteins travel through the pores, which delays their progress compared to large proteins. Elute with
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7.05_Problem_Set_1_answers - 7.05 Problem Set 1 Answers 1.)...

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