7.05Exam4ReviewPart1

7.05Exam4ReviewPart1 - Page 1 of 28 7.05 Exam 4 Review...

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Page 1 of 28 7.05 Exam 4 – Review Handout Part 1 Lecture 27-30: Pentose Metabolism, Lipids and FA, Oxidative Phosphorylation, Photosynthesis, Amino Acids, Urea Cycle Everything you'll ever need : http://www.jiwani.net/biochem.html Suggestions: Redraw and understand all mechanisms on this handout Come to review session where we will do the review problems (provided at the end of this review) [BRING HANDOUT] Review all problems provided in this handout and understand answers provided What you need on your sheets: Pentose mechanism o Oxidation Step (NADPH produced) o Mechanisms of transaldolase and transketose o Good way to produce ribose-5-P for RNA synthesis
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Page 2 of 28 Pentose Cycle Practice Problems
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Page 5 of 28 Biosynthesis of Phospholipids o Follow chart, but basically: o Activate => oxidize/convert =>hydrate => oxide => cleave
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Page 6 of 28 B. Fatty Acid Biosynthesis o Carboxylate, elongate, reduce, dehydrate, reduce
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Page 7 of 28 Fatty Acid Practice Problems 1999 Exam 3
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Page 8 of 28 1999 Exam 3
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Page 9 of 28 1998 Exam 3
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Page 10 of 28 2000 Exam 3
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Page 12 of 28 1995 Exam 3
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Page 13 of 28 Oxidative Phosphorylation Simplified Version A major source of cellular energy production, in the form of ATP, is derived from the proton motive force supplied to mitochondrial ATP synthase. The main driver of the proton gradient across the inner mitochondrial membrane is the electron transport system. NADH and FADH2 produced by glycolysis, fatty acid oxidation and the TCA cycle serve as a electron donors and cofactors for the protein complexes involved in the electron transport system. The net result is an influx of protons into the intermembrane space of the mitochondria and the production of water as a byproduct in the inner mitochondrial membrane. 2 H + + 2 e + + 1/2 O 2 H 2 O + energy There are four membrane-bound protein complexes that participate in the electron transport system. Complex I is a NADH dehydrogenase (or NADH-Coenzyme Q Reductase) composed of FMN, Coenzyme Q and Fe-S clusters. Using NADH as the initial electron donor, complex I generates a net result of 4 protons transferred from the matrix to the intermembrane space of the mitochondria, and the transfer of two electrons and two protons to reduce coenzyme Q located outside the complex within the inner mitochondrial membrane.
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Page 14 of 28 Complex II
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7.05Exam4ReviewPart1 - Page 1 of 28 7.05 Exam 4 Review...

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