2008_ps7a

2008_ps7a - with the organism. Draw a diagram of this...

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Problem Set 8 Solutions – Oxidative Phosphorylation, Photosynthesis, Assimilation of CO 2 Oxidative Phosphorylation 1) Explain the purpose of O 2 in animal metabolism. O 2 is the ultimate electron acceptor in the electron transport chain. 2) One night, while you were trudging through another round of tissue culture, you discover some raggedy cells. Upon (extremely close) examination, you find that in some of the mitochondria, the pH of the matrix is equal to the pH of the intermembrane. What do you think is physically responsible for this phenomenon? What is the effect of this mutation on ATP production? The intermembrane is now permeable to H + , thus H + flow is not coupled with the ATP synthase. Without an electrochemical gradient, the ATP synthase cannot generate more ATP. 3) The next day, your friend who works across the bay discover a type of single-cellular organism that does not have mitochondria, yet oxidative phosphorylation still takes place
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Unformatted text preview: with the organism. Draw a diagram of this amazing organism and indicate where the ATP synthase, freshly generated ATP, and oxidative phosphorylation complexes are located. 4) Describe the two consequences of a mutation where FMN is no longer present in the inner membrane of the mitochondria. Without FMN, the FMN FMNH 2 reduction reaction cannot occur which means that NADH cannot be oxidized back to the NAD, effectively stopping glycolysis and the TCA cycle. Outer membrane Inner membrane Nucleus ATP synthas ADP ATP Oxidative Phospohrylation Complexes Photosynthesis/Assimilation of CO 2 6) If a plant is grown in the presence of 14 C-labelled CO 2 , which C atoms of the resulting fructose-6-phosphate will emerge with the radioactive label after a single round of the Calvin Cycle? Which C atoms will be labeled after a second round of the cycle?...
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2008_ps7a - with the organism. Draw a diagram of this...

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