7.05Pset4

7.05Pset4 - 7.05 Spring 2008 Problem Set #4: Lectures 14-19...

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7.05 – Spring 2008 Problem Set #4: Lectures 14-19 Problem 1: Riboswitches – 2006 Exam 2
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Problem 2 – Post-translational Modifications a) Thalassemia is an inherited autosomal recessive blood disease. In thalassemia, the genetic defect results in reduced rate of synthesis of one of the globin chains that make up hemoglobin. To locate the site of mutation, you must first separate mRNA from other types of RNA in a eukaryotic cells. What is one separation technique you can use? Many answers possible. Can attach an oligo(dT) or oligo(U) sequence to an inert support to create an affinity column. When RNA is passed through the column, only poly(A)- containing RNA will be retained. b) You have successfully located an mRNA segment of interest. In one type of mutation leading to a form of thalassemia, the mutation of a single base (G to A) generates a new 3’ splice site (boxed) akin to the normal one (bolded) but father upstream. 5’ C C T A T T G G T C T A T T T T C C A C C C
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This note was uploaded on 02/06/2009 for the course 7 7.05 taught by Professor Unknown during the Spring '09 term at MIT.

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7.05Pset4 - 7.05 Spring 2008 Problem Set #4: Lectures 14-19...

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