noname

noname - Page 1 of 9 Recitation #5 March 10, 2008 Lecture...

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Page 1 of 9 Recitation #5 – March 10, 2008 Lecture 9-12: DNA, RNA, replication, transcription, and translation Contact Information: Patricia Zheng Email: pzheng@mit.edu
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Page 5 of 9 Practice Problems Problem 1: 2003 Exam I
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Page 7 of 9 Problem 2: 1998 Exam 1 Protein engineering refers to an area of structural biology in which scientists try to alter the properties of proteins. Suppose you were able to engineer a DNA polymerase that contained a modified 3’ Æ 5’ exonuclease activity with the following property: the exonuclease activity required that the 3’-nucleotide was part of a double helix. What would you expect the properties of this engineered enzyme to be, in terms of fidelity and overall speed of replication, as compared to the otherwise identical DNA polymerase that completely lacks 3’ Æ 5’ exonuclease activity? Explain. The engineered polymerase will cut out properly inserted base pairs and leave mistakes in place. The resulting DNA will have almost 100% mistakes. The polymerase that lacks the exonuclease activity will remove nothing, which means about 1 out of 10^4
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noname - Page 1 of 9 Recitation #5 March 10, 2008 Lecture...

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